|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM241033438 |
| 003 |
DE-627 |
| 005 |
20231224123356.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/eru324
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0803.xml
|
| 035 |
|
|
|a (DE-627)NLM241033438
|
| 035 |
|
|
|a (NLM)25129131
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Irfan, Mohammad
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Insights into transcriptional regulation of β-D-N-acetylhexosaminidase, an N-glycan-processing enzyme involved in ripening-associated fruit softening
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 08.07.2015
|
| 500 |
|
|
|a Date Revised 07.12.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme, β-D-N-acetylhexosaminidase (β-Hex), plays an important role in the ripening-associated fruit-softening process. However, the regulation of fruit ripening-specific expression of β-Hex is not well understood. We have identified and functionally characterized the fruit ripening-specific promoter of β-Hex and provided insights into its transcriptional regulation during fruit ripening. Our results demonstrate that RIPENING INHIBITOR (RIN), a global fruit ripening regulator, and ABSCISIC ACID STRESS RIPENING 1 (SlASR1), a poorly characterized ripening-related protein, are the transcriptional regulators of β-Hex. Both RIN and SlASR1 directly bound to the β-Hex promoter fragments containing CArG and C₂₋₃(C/G)A cis-acting elements, the binding sites for RIN and SlASR1, respectively. Moreover, β-Hex expression/promoter activity in tomato fruits was downregulated once expression of either RIN or SlASR1 was suppressed; indicating that RIN and SlASR1 positively regulate the transcription of β-Hex during fruit ripening. Interestingly, RIN could also bind to the SlASR1 promoter, which contains several CArG cis-acting elements, and SlASR1 expression was suppressed in rin mutant fruits, indicating that RIN also acts as a positive regulator of SlASR1 expression during fruit ripening. Taken together, these results suggest that RIN, both directly and indirectly, through SlASR1, regulates the transcription of β-Hex during fruit ripening. The fruit ripening-specific promoter of β-Hex could be a useful tool in regulating gene expression during fruit ripening
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Fruit ripening
|
| 650 |
|
4 |
|a RIN
|
| 650 |
|
4 |
|a SlASR1
|
| 650 |
|
4 |
|a fruit ripening-specific promoter
|
| 650 |
|
4 |
|a transcriptional regulation.
|
| 650 |
|
4 |
|a β-Hex
|
| 650 |
|
7 |
|a Ethylenes
|2 NLM
|
| 650 |
|
7 |
|a Glucans
|2 NLM
|
| 650 |
|
7 |
|a MADS Domain Proteins
|2 NLM
|
| 650 |
|
7 |
|a Plant Growth Regulators
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Abscisic Acid
|2 NLM
|
| 650 |
|
7 |
|a 72S9A8J5GW
|2 NLM
|
| 650 |
|
7 |
|a beta-N-Acetylhexosaminidases
|2 NLM
|
| 650 |
|
7 |
|a EC 3.2.1.52
|2 NLM
|
| 700 |
1 |
|
|a Ghosh, Sumit
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kumar, Vinay
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chakraborty, Niranjan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chakraborty, Subhra
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Datta, Asis
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 20 vom: 16. Nov., Seite 5835-48
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:65
|g year:2014
|g number:20
|g day:16
|g month:11
|g pages:5835-48
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/eru324
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 65
|j 2014
|e 20
|b 16
|c 11
|h 5835-48
|