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231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la501876m
|2 doi
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|a pubmed24n0798.xml
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|a (DE-627)NLM239636155
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|a (NLM)24976495
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Qiong
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Europium-complex-grafted polymer dots for amplified quenching and cellular imaging applications
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|c 2014
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 11.05.2015
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|a Date Revised 22.07.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We report on a europium-complex-grafted polymer for preparing stable nanoparticle probes with high luminescence brightness, narrow emission bandwidth, and long luminescence lifetimes. A Eu complex bearing an amino group was used to react with a functional copolymer poly(styrene-co-maleic anhydride) by the spontaneous amidation reaction, producing the polymer grafted with Eu complexes in the side chains. The Eu-complex-grafted polymer was further used to prepare Eu-complex-grafted polymer dots (Pdots) and Eu-complex-blended poly(9-vinylcarbazole) composite Pdots, which showed improved colloidal stability as compared to those directly doped with Eu-complex molecules. Both types of Pdots can be efficiently quenched by a nile blue dye, exhibiting much lower detection limit and higher quenching sensitivity as compared to free Eu-complex molecules. Steady-state spectroscopy and time-resolved decay dynamics suggest the quenching mechanism is via efficient fluorescence resonance energy transfer from the Eu complex inside a Pdot to surface dye molecules. The amplified quenching in Eu-complex Pdots, together with efficient cell uptake and specific cell surface labeling observed in mammalian cells, suggests their potential applications in time-resolved bioassays and cellular imaging
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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7 |
|a Maleates
|2 NLM
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|a Polymers
|2 NLM
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|a Polystyrenes
|2 NLM
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|a poly(styrene-co-maleic anhydride)
|2 NLM
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|a Europium
|2 NLM
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| 650 |
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|a 444W947O8O
|2 NLM
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| 700 |
1 |
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|a Zhang, Jianan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Wei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Jiangbo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Changfeng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qin, Weiping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chiu, Daniel T
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 28 vom: 22. Juli, Seite 8607-14
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:30
|g year:2014
|g number:28
|g day:22
|g month:07
|g pages:8607-14
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|u http://dx.doi.org/10.1021/la501876m
|3 Volltext
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