Europium-complex-grafted polymer dots for amplified quenching and cellular imaging applications
We report on a europium-complex-grafted polymer for preparing stable nanoparticle probes with high luminescence brightness, narrow emission bandwidth, and long luminescence lifetimes. A Eu complex bearing an amino group was used to react with a functional copolymer poly(styrene-co-maleic anhydride)...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 30(2014), 28 vom: 22. Juli, Seite 8607-14 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Maleates Polymers Polystyrenes poly(styrene-co-maleic anhydride) Europium 444W947O8O |
| Zusammenfassung: | We report on a europium-complex-grafted polymer for preparing stable nanoparticle probes with high luminescence brightness, narrow emission bandwidth, and long luminescence lifetimes. A Eu complex bearing an amino group was used to react with a functional copolymer poly(styrene-co-maleic anhydride) by the spontaneous amidation reaction, producing the polymer grafted with Eu complexes in the side chains. The Eu-complex-grafted polymer was further used to prepare Eu-complex-grafted polymer dots (Pdots) and Eu-complex-blended poly(9-vinylcarbazole) composite Pdots, which showed improved colloidal stability as compared to those directly doped with Eu-complex molecules. Both types of Pdots can be efficiently quenched by a nile blue dye, exhibiting much lower detection limit and higher quenching sensitivity as compared to free Eu-complex molecules. Steady-state spectroscopy and time-resolved decay dynamics suggest the quenching mechanism is via efficient fluorescence resonance energy transfer from the Eu complex inside a Pdot to surface dye molecules. The amplified quenching in Eu-complex Pdots, together with efficient cell uptake and specific cell surface labeling observed in mammalian cells, suggests their potential applications in time-resolved bioassays and cellular imaging |
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| Beschreibung: | Date Completed 11.05.2015 Date Revised 22.07.2014 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la501876m |