Simultaneous quantification of alternatively spliced transcripts in a single droplet digital PCR reaction
Human telomerase reverse transcriptase (hTERT) is an essential component required for telomerase activity and telomere maintenance. Several alternatively spliced forms of hTERT mRNA have been reported in human primary and tumor cells. Currently, however, there is no sensitive and accurate method for...
Ausführliche Beschreibung
Bibliographische Detailangaben
Veröffentlicht in: | BioTechniques. - 1993. - 56(2014), 6 vom: 13. Juni, Seite 319-25
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1. Verfasser: |
Sun, Bing
(VerfasserIn) |
Weitere Verfasser: |
Tao, Lian,
Zheng, Yun-Ling |
Format: | Online-Aufsatz
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Sprache: | English |
Veröffentlicht: |
2014
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Zugriff auf das übergeordnete Werk: | BioTechniques
|
Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
RNA alternative splicing
ddPCR
droplet digital PCR
hTERT
qPCR
real-time PCR
telomerase
mehr...
telomere
DNA Primers
DNA-Binding Proteins
RNA, Messenger
TERT protein, human
EC 2.7.7.49
Telomerase |