Simultaneous quantification of alternatively spliced transcripts in a single droplet digital PCR reaction

Human telomerase reverse transcriptase (hTERT) is an essential component required for telomerase activity and telomere maintenance. Several alternatively spliced forms of hTERT mRNA have been reported in human primary and tumor cells. Currently, however, there is no sensitive and accurate method for...

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Veröffentlicht in:BioTechniques. - 1993. - 56(2014), 6 vom: 13. Juni, Seite 319-25
1. Verfasser: Sun, Bing (VerfasserIn)
Weitere Verfasser: Tao, Lian, Zheng, Yun-Ling
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't RNA alternative splicing ddPCR droplet digital PCR hTERT qPCR real-time PCR telomerase mehr... telomere DNA Primers DNA-Binding Proteins RNA, Messenger TERT protein, human EC 2.7.7.49 Telomerase