Relationship between permeability glycoprotein (P-gp) gene expression and enrofloxacin metabolism in Nile Tilapia
The aim of this study was to analyze the influence of permeability glycoprotein (P-gp) gene expression on enrofloxacin (ENR) metabolism in aquatic animals. Nile Tilapia Oreochomis niloticus were fed different doses of ENR ranging from 0 to 80 mg/kg. The P-gp gene expression levels were determined by...
| Veröffentlicht in: | Journal of aquatic animal health. - 1998. - 26(2014), 2 vom: 28. Juni, Seite 59-65 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | Journal of aquatic animal health |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't ATP Binding Cassette Transporter, Subfamily B, Member 1 Anti-Bacterial Agents Fluoroquinolones Enrofloxacin 3DX3XEK1BN |
| Zusammenfassung: | The aim of this study was to analyze the influence of permeability glycoprotein (P-gp) gene expression on enrofloxacin (ENR) metabolism in aquatic animals. Nile Tilapia Oreochomis niloticus were fed different doses of ENR ranging from 0 to 80 mg/kg. The P-gp gene expression levels were determined by quantitative real-time PCR (qRT-PCR) at indicated time points after drug administration. Drug metabolism was determined by HPLC. The P-gp gene expression in liver and kidney was greatly enhanced 30 min after ENR administration at 40 mg/kg, peaked 3 h after drug administration, and then gradually decreased. Thirty minutes after a single oral administration of ENR (0, 20, 40, or 80 mg/kg), the P-gp gene expression increased in a dose-dependent manner. The P-gp gene expression levels in the kidney were significantly higher than those in the liver. Additionally, the metabolic rate of ENR in kidney was more rapid than that in liver. Furthermore, a close correlation was found between P-gp gene expression and ENR concentrations. These results suggest that P-gp may be involved in the ENR metabolism process in Nile Tilapia, providing a novel model for the potential utility of gene expression and drug metabolism studies in aquatic animals |
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| Beschreibung: | Date Completed 04.09.2014 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1548-8667 |
| DOI: | 10.1080/08997659.2013.860059 |