Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning

The recombinant adeno-associated virus (rAAV) has proven to be an efficient and attractive tool for targeted genome engineering. Here we present a novel method employing the Golden Gate cloning strategy for fast and efficient construction of rAAV-based gene knockout or single-nucleotide knockin vect...

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Publié dans:	BioTechniques. - 1993. - 56(2014), 5 vom: 09. Mai, Seite 263-8
Auteur principal:	Luo, Yonglun (Auteur)
Autres auteurs:	Lin, Lin, Bolund, Lars, Sørensen, Charlotte Brandt
Format:	Article en ligne
Langue:	English
Publié:	2014
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, Non-U.S. Gov't Golden Gate cloning gene targeting p53 rAAV tau Cinnamates Mapt protein, mouse tau Proteins plus... Hygromycin B 3XQ2233B0B hygromycin A 3YJY415DDI Neomycin I16QD7X297