Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning
The recombinant adeno-associated virus (rAAV) has proven to be an efficient and attractive tool for targeted genome engineering. Here we present a novel method employing the Golden Gate cloning strategy for fast and efficient construction of rAAV-based gene knockout or single-nucleotide knockin vect...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 56(2014), 5 vom: 09. Mai, Seite 263-8
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| 1. Verfasser: |
Luo, Yonglun
(VerfasserIn) |
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| Weitere Verfasser: |
Lin, Lin,
Bolund, Lars,
Sørensen, Charlotte Brandt |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Golden Gate cloning
gene targeting
p53
rAAV
tau
Cinnamates
Mapt protein, mouse
tau Proteins
mehr...
Hygromycin B
3XQ2233B0B
hygromycin A
3YJY415DDI
Neomycin
I16QD7X297 |
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