Bcl-2 suppresses activation of VPEs by inhibiting cytosolic Ca²⁺ level with elevated K⁺ efflux in NaCl-induced PCD in rice
Copyright © 2014 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 80(2014) vom: 30. Juli, Seite 168-75 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Bcl-2 Cytosolic Ca(2+) K(+) efflux NaCl stress Programmed cell death Transgenic rice Vacuolar processing enzyme Proto-Oncogene Proteins c-bcl-2 mehr... |
| Zusammenfassung: | Copyright © 2014 Elsevier Masson SAS. All rights reserved. Bcl-2 is one of the most important antiapoptotic members in mammals and prevents many forms of apoptosis in a variety of cell types. Our previous study revealed that overexpression of Bcl-2 significantly suppressed H2O2/NaCl-induced programmed cell death via inhibiting the transcriptional activation of OsVPE2 and OsVPE3 in transgenic rice. However, Ca(2+) and K(+) homeostasis of this process remains largely unknown. In the present study, we investigate whether nonselective cation channels (NSCC) blockers affect Bcl-2 function in rice under salt stress and how Bcl-2 affects ion homeostasis in salt stress-induced PCD. The results showed that overexpression of Bcl-2 significantly decreased transient elevations in the cytosolic Ca(2+) levels, inhibited NaCl-induced K(+) efflux but not H(+) efflux across the plasma membrane, and further suppressed the expression levels of OsVPE2 and OsVPE3, leading to the inhibition of salt-induced PCD and increase of tolerance to salt stress in transgenic rice. During the NaCl-induced PCD, the effects of a NSCC blocker La(3+) on ion homeostasis and VPEs expression in wild-type were similar to the effects of Bcl-2 overexpression in transgenic line. However, a synergistic effect of Bcl-2 and La(3+) was not obviously detectable. Our results suggested that Bcl-2 played an important role in suppression of NaCl-induced PCD by disruption of ion homeostasis, providing an insight into the mechanistic study of plant VPEs, cytosolic Ca(2+) level and K(+) efflux |
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| Beschreibung: | Date Completed 15.01.2015 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2014.04.002 |