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231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la4049852
|2 doi
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|a pubmed24n0789.xml
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|a (NLM)24673363
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Ali, Md Azahar
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Lipid-lipid interactions in aminated reduced graphene oxide interface for biosensing application
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|c 2014
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 10.04.2015
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|a Date Revised 15.04.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A label-free biosensor based on antiapolipoprotein B 100 functionalized-aminated reduced graphene oxide interface has been fabricated for detection of low density lipoprotein (LDL or lipid) cholesterol. The aminated reduced graphene oxide (NH2-rGO) based electrode surface is covalently functionalized with antiapolipoprotein B 100 (AAB or lipid) using EDC/NHS coupling chemistry. The lipid-lipid interactions at the NH2-rGO electrode surface have been investigated using electrochemical impedance spectroscopic technique. The structural and morphological investigations of NH2-rGO based immunosensor have been accomplished via transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, UV-visible, and electrochemical techniques. The impedimetric response of the proposed immunosensor shows excellent sensitivity (612 Ω mg(-1) dL cm(-2)), a response time of 250 s, and a low detection limit of 5 mg/dL of LDL molecules. The association, dissociation, and equilibrium rate constants for this immunoelectrode are found to be 1.66 M(-1) s(-1), 0.6 s(-1), and 2.77 M(-1), respectively. The long-term stability and excellent reproducibility of the proposed immunosensor indicates a suitable platform for detection of LDL or lipid molecules. This immunosensor provides an efficient platform for analysis of the antigen-antibody interactions of lipid molecules
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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7 |
|a Lipids
|2 NLM
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| 650 |
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|a Oxides
|2 NLM
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|a Graphite
|2 NLM
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|a 7782-42-5
|2 NLM
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| 700 |
1 |
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|a Kamil Reza, K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Srivastava, Saurabh
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Agrawal, Ved Varun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a John, Renu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Malhotra, Bansi Dhar
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 14 vom: 15. Apr., Seite 4192-201
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:30
|g year:2014
|g number:14
|g day:15
|g month:04
|g pages:4192-201
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|u http://dx.doi.org/10.1021/la4049852
|3 Volltext
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