How do background ozone concentrations affect the biosynthesis of rosmarinic acid in Melissa officinalis?

Bibliographische Detailangaben
Veröffentlicht in:	Journal of plant physiology. - 1979. - 171(2014), 5 vom: 01. März, Seite 35-41
1. Verfasser:	Döring, Anne S (VerfasserIn)
Weitere Verfasser:	Pellegrini, Elisa, Della Batola, Michele, Nali, Cristina, Lorenzini, Giacomo, Petersen, Maike
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	Journal of plant physiology
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Air pollution Expression analysis Lemon balm Rosmarinic acid qRT-PCR Cinnamates Depsides Plant Proteins mehr... Ozone 66H7ZZK23N

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier GmbH. All rights reserved. Lemon balm (Melissa officinalis; Lamiaceae) plants were exposed to background ozone (O3) dosages (80ppb for 5h), because high background levels of O3 are considered to be as harmful as episodic O3 peaks. Immediately at the end of fumigation the plants appeared visually symptomless, but necrotic lesions were observed later. The biosynthesis of rosmarinic acid (RA) comprises eight enzymes, among them phenylalanine ammonia-lyase (PAL), 4-coumarate:coenzyme A ligase (4CL), tyrosine aminotransferase (TAT) and rosmarinic acid synthase (RAS). The transcript levels of these genes have been investigated by quantitative RT-PCR. There was a quick up-regulation of all genes at 3h of O3 exposure, but at 24h from beginning of exposure (FBE) only RAS and PAL were up-regulated. The specific activity of RAS was closely correlated with a decrease of RA concentration in lemon balm leaves. The specific activity of PAL increased at 12h FBE to 163% in comparison to control levels. This work provides insight into the effect of O3 stress on the formation of the main phenolic ingredient of the pharmaceutically important plant M. officinalis
Beschreibung:	Date Completed 24.09.2014 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1618-1328
DOI:	10.1016/j.jplph.2013.11.005