Proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions

Proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions

Copyright © 2014 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 75(2014) vom: 15. Feb., Seite 96-104
1. Verfasser: Wang, Xiaoli (VerfasserIn)
Weitere Verfasser: Tang, Dongmei, Huang, Danfeng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 2-DE 3-[(3-cholamido-propyl)-dimethylammonio]-1-propanesulfonate Amino acid CA CAN CBB CCS CHAPS mehr... Coomassie brilliant blue DTT FNR GR-RBP GS Gly-rich RNA-binding protein Glycine IPG MALDI-TOF MS Nitrate PR Pakchoi Pn Proteomics ROS RSS Reactive oxygen species Rubisco S-adenosylmethionine synthase isozyme SAMS TCTP acetonitrile carbonic anhydrase copper chaperone dithiothreitol ferredoxin-NADP(+) reductase glutamine synthetase immobilized pH gradient mass spectrometry matrix-assisted laser desorption and ionization time-of-flight net photosynthesis rate putative pathogenesis-related protein reactive sulfur species ribulose-1,5-bisphosphate carboxylase/oxygenase translationally-controlled tumor protein homolog two-dimensional gel electrophoresis Plant Proteins Proteome Adenosine Triphosphate 8L70Q75FXE Nitrogen N762921K75 TE7660XO1C
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100 1 |a Wang, Xiaoli  |e verfasserin  |4 aut 
245 1 0 |a Proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions 
264 1 |c 2014 
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338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 29.09.2014 
500 |a Date Revised 30.09.2020 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2014 Elsevier Masson SAS. All rights reserved. 
520 |a The physiological and differential proteomic responses of pakchoi leaves and roots to glycine-nitrogen (Gly-N) treatments were determined. Two pakchoi (Brassica campestris ssp. chinensis L. Makino. var. communis Tsen et Lee) cultivars, 'Huawang' and 'Wuyueman', were grown under sterile hydroponic conditions with different N forms (Gly-N and nitrate-N). Gly-N-treated pakchoi exhibited decreased fresh weights, total N uptake, leaf areas, and net photosynthetic rates than those treated with nitrate-N. Differentially regulated proteins were selected after image analysis and identified using MALDI-TOF MS. A total of 23 proteins was up- or down-regulated following Gly-N treatment. These spots are involved in several processes, such as energy synthesis, N metabolism, photosynthesis, and active antioxidant defense mechanisms, that could enhance plant adaptation to Gly-N. The superior Gly tolerance of 'Huawang' was predominantly associated with a less severe down-regulation of proteins that are involved in the electron transport chain and N metabolism. Other factors could include less ribulose-1,5-bisphosphate carboxylase/oxygenase turnover or a higher up-regulation of stress defense proteins. These characteristics demonstrated that maintaining ATP synthesis, N metabolism, photosynthesis, and active defense mechanisms play a critical role in pakchoi adaptation to Gly-N 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a 2-DE 
650 4 |a 3-[(3-cholamido-propyl)-dimethylammonio]-1-propanesulfonate 
650 4 |a Amino acid 
650 4 |a CA 
650 4 |a CAN 
650 4 |a CBB 
650 4 |a CCS 
650 4 |a CHAPS 
650 4 |a Coomassie brilliant blue 
650 4 |a DTT 
650 4 |a FNR 
650 4 |a GR-RBP 
650 4 |a GS 
650 4 |a Gly-rich RNA-binding protein 
650 4 |a Glycine 
650 4 |a IPG 
650 4 |a MALDI-TOF 
650 4 |a MS 
650 4 |a Nitrate 
650 4 |a PR 
650 4 |a Pakchoi 
650 4 |a Pn 
650 4 |a Proteomics 
650 4 |a ROS 
650 4 |a RSS 
650 4 |a Reactive oxygen species 
650 4 |a Rubisco 
650 4 |a S-adenosylmethionine synthase isozyme 
650 4 |a SAMS 
650 4 |a TCTP 
650 4 |a acetonitrile 
650 4 |a carbonic anhydrase 
650 4 |a copper chaperone 
650 4 |a dithiothreitol 
650 4 |a ferredoxin-NADP(+) reductase 
650 4 |a glutamine synthetase 
650 4 |a immobilized pH gradient 
650 4 |a mass spectrometry 
650 4 |a matrix-assisted laser desorption and ionization time-of-flight 
650 4 |a net photosynthesis rate 
650 4 |a putative pathogenesis-related protein 
650 4 |a reactive sulfur species 
650 4 |a ribulose-1,5-bisphosphate carboxylase/oxygenase 
650 4 |a translationally-controlled tumor protein homolog 
650 4 |a two-dimensional gel electrophoresis 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Proteome  |2 NLM 
650 7 |a Adenosine Triphosphate  |2 NLM 
650 7 |a 8L70Q75FXE  |2 NLM 
650 7 |a Nitrogen  |2 NLM 
650 7 |a N762921K75  |2 NLM 
650 7 |a Glycine  |2 NLM 
650 7 |a TE7660XO1C  |2 NLM 
700 1 |a Tang, Dongmei  |e verfasserin  |4 aut 
700 1 |a Huang, Danfeng  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 75(2014) vom: 15. Feb., Seite 96-104  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:75  |g year:2014  |g day:15  |g month:02  |g pages:96-104 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2013.12.012  |3 Volltext 
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