Proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 75(2014) vom: 15. Feb., Seite 96-104
1. Verfasser:	Wang, Xiaoli (VerfasserIn)
Weitere Verfasser:	Tang, Dongmei, Huang, Danfeng
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't 2-DE 3-[(3-cholamido-propyl)-dimethylammonio]-1-propanesulfonate Amino acid CA CAN CBB CCS CHAPS mehr... Coomassie brilliant blue DTT FNR GR-RBP GS Gly-rich RNA-binding protein Glycine IPG MALDI-TOF MS Nitrate PR Pakchoi Pn Proteomics ROS RSS Reactive oxygen species Rubisco S-adenosylmethionine synthase isozyme SAMS TCTP acetonitrile carbonic anhydrase copper chaperone dithiothreitol ferredoxin-NADP(+) reductase glutamine synthetase immobilized pH gradient mass spectrometry matrix-assisted laser desorption and ionization time-of-flight net photosynthesis rate putative pathogenesis-related protein reactive sulfur species ribulose-1,5-bisphosphate carboxylase/oxygenase translationally-controlled tumor protein homolog two-dimensional gel electrophoresis Plant Proteins Proteome Adenosine Triphosphate 8L70Q75FXE Nitrogen N762921K75 TE7660XO1C

Beschreibung
Zusammenfassung:	Copyright © 2014 Elsevier Masson SAS. All rights reserved. The physiological and differential proteomic responses of pakchoi leaves and roots to glycine-nitrogen (Gly-N) treatments were determined. Two pakchoi (Brassica campestris ssp. chinensis L. Makino. var. communis Tsen et Lee) cultivars, 'Huawang' and 'Wuyueman', were grown under sterile hydroponic conditions with different N forms (Gly-N and nitrate-N). Gly-N-treated pakchoi exhibited decreased fresh weights, total N uptake, leaf areas, and net photosynthetic rates than those treated with nitrate-N. Differentially regulated proteins were selected after image analysis and identified using MALDI-TOF MS. A total of 23 proteins was up- or down-regulated following Gly-N treatment. These spots are involved in several processes, such as energy synthesis, N metabolism, photosynthesis, and active antioxidant defense mechanisms, that could enhance plant adaptation to Gly-N. The superior Gly tolerance of 'Huawang' was predominantly associated with a less severe down-regulation of proteins that are involved in the electron transport chain and N metabolism. Other factors could include less ribulose-1,5-bisphosphate carboxylase/oxygenase turnover or a higher up-regulation of stress defense proteins. These characteristics demonstrated that maintaining ATP synthesis, N metabolism, photosynthesis, and active defense mechanisms play a critical role in pakchoi adaptation to Gly-N
Beschreibung:	Date Completed 29.09.2014 Date Revised 09.01.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.12.012