Carbon-nitrogen ratio and in vitro assimilate partitioning patterns in Cyrtanthus guthrieae L

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 74(2014) vom: 03. Jan., Seite 246-54
1. Verfasser: Ncube, Bhekumthetho (VerfasserIn)
Weitere Verfasser: Finnie, Jeffrey F, Van Staden, Johannes
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 6-benzyladenine AE Assimilate BA BCG Bromocresol green CNB CTE mehr... Carbon Cyrtanthus guthrieae DDI DM DMRT Duncan's multiple range test FM GAE GE LE LSD MS Metabolites Murashige and Skoog NAA Nitrogen Nutrients PCM Polyphenol RGR atropine equivalents carbon/nitrogen balance catechin equivalents distilled and deionised dry mass fresh mass gallic acid equivalents glucose equivalents least significant difference leucocyanidin equivalents naphthalene acetic acid protein competition model relative growth rate 7440-44-0 N762921K75
Beschreibung
Zusammenfassung:Copyright © 2013 Elsevier Masson SAS. All rights reserved.
In response to variations in nutritional composition of the growth medium, plants often adjust their metabolism and progressively alter their growth patterns. Carbon (C) and nitrogen (N) constitute the major plant nutritional components influencing plant growth and development patterns. This study examined the growth dynamics and patterns of assimilate partitioning to primary and secondary metabolites in response to varying levels and combinations of C and N in the culture media of Cyrtanthus guthrieae. In vitro callus-derived C. guthrieae plantlets were cultured on solid Murashige and Skoog (MS) media with different concentrations and combinations of C and N. Relative growth rate (RGR) increased proportionally with an increase in C concentrations up to 88 mM sucrose (0.58 d(-1)) beyond which it was hardly influenced by further increases in C. Growth was also significantly favoured in media with high concentrations of N at all C concentrations tested. In C-limited media regimes with growth saturating N conditions, alkaloid accumulation became favoured while polyphenol content increased with an increase in C levels in the medium, a characteristic pattern that appeared to be less influenced by the amount of N. Of the primary metabolites, only proteins showed small significant variations across different media treatments, with starch and soluble sugars increasing proportionately with C levels. In the medium with a high sucrose concentration (175 mM), soluble sugars, amino acids and polyphenols increased markedly, possibly as an adaptive response to the reduced osmotic potential in the media and/or a storage mechanism for excess C and N reserves in the media. From a medicinal perspective, with regard to polyphenolic compounds in C. guthrieae, growth medium conditions that allow for high levels of C pools in the tissue would thus be favourable for the enhanced synthesis of this group of compounds. The medium conditions with 175 mM sucrose and 10.3 mM NH4NO3 gave the highest total polyphenols, flavonoids and proanthocyanidins with a moderate growth rate
Beschreibung:Date Completed 22.09.2014
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2013.11.007