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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12086
|2 doi
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|a pubmed25n0778.xml
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|a (NLM)24320784
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|a DE-627
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|e rakwb
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|a eng
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1 |
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|a Masserdotti, Carlo
|e verfasserin
|4 aut
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|a Proportion of mast cells in normal canine hepatic cytologic specimens
|b comparison of 2 staining methods
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 12.05.2015
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|a Date Revised 20.10.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2013 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the normal proportion of these cells in cytologic specimens from normal livers was not previously established
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|a OBJECTIVES: The objective of this study was to determine the normal proportion of hepatic mast cells in cytologic specimens from normal canine liver by the use and comparison of the May-Grünwald-Giemsa and the Toluidine Blue staining methods
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|a METHODS: The cytologic specimens obtained from fine-needle aspirates of livers of 13 healthy dogs were investigated by counting of hepatocytes and mast cells per high-power field (HPF, x40 objective). The ratio of mast cells/100 hepatocytes was then compared between the 2 staining methods
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| 520 |
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|a RESULTS: A median of 0.17 (range: 0-0.33) mast cells/100 hepatocytes and of 0.47 (range: 0-0.86) mast cells/100 hepatocytes was obtained with May-Grünwald-Giemsa and Toluidine Blue stains, respectively
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|a CONCLUSIONS: Toluidine Blue stain detects more hepatic mast cells than May-Grunwald-Giemsa in normal canine livers. With both stains, < 1% mast cells were detected in normal canine liver samples
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|a Comparative Study
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|a Journal Article
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|a Liver
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|a mast cell granules
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|a mastocytoma staging
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|a mastocytosis
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|a Coloring Agents
|2 NLM
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|a May-Grunwald Giemsa
|2 NLM
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|a Tolonium Chloride
|2 NLM
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|a 15XUH0X66N
|2 NLM
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|a Methylene Blue
|2 NLM
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|a T42P99266K
|2 NLM
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| 650 |
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|a Eosine Yellowish-(YS)
|2 NLM
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| 650 |
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|a TDQ283MPCW
|2 NLM
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| 773 |
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|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 42(2013), 4 vom: 23. Dez., Seite 522-5
|w (DE-627)NLM098159984
|x 1939-165X
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1 |
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|g volume:42
|g year:2013
|g number:4
|g day:23
|g month:12
|g pages:522-5
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|u http://dx.doi.org/10.1111/vcp.12086
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