Proportion of mast cells in normal canine hepatic cytologic specimens : comparison of 2 staining methods
© 2013 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.
| Publié dans: | Veterinary clinical pathology. - 1975. - 42(2013), 4 vom: 23. Dez., Seite 522-5 |
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| Auteur principal: | |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2013
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| Accès à la collection: | Veterinary clinical pathology |
| Sujets: | Comparative Study Journal Article Liver mast cell granules mastocytoma staging mastocytosis Coloring Agents May-Grunwald Giemsa Tolonium Chloride 15XUH0X66N plus... |
| Résumé: | © 2013 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology. BACKGROUND: Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the normal proportion of these cells in cytologic specimens from normal livers was not previously established OBJECTIVES: The objective of this study was to determine the normal proportion of hepatic mast cells in cytologic specimens from normal canine liver by the use and comparison of the May-Grünwald-Giemsa and the Toluidine Blue staining methods METHODS: The cytologic specimens obtained from fine-needle aspirates of livers of 13 healthy dogs were investigated by counting of hepatocytes and mast cells per high-power field (HPF, x40 objective). The ratio of mast cells/100 hepatocytes was then compared between the 2 staining methods RESULTS: A median of 0.17 (range: 0-0.33) mast cells/100 hepatocytes and of 0.47 (range: 0-0.86) mast cells/100 hepatocytes was obtained with May-Grünwald-Giemsa and Toluidine Blue stains, respectively CONCLUSIONS: Toluidine Blue stain detects more hepatic mast cells than May-Grunwald-Giemsa in normal canine livers. With both stains, < 1% mast cells were detected in normal canine liver samples |
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| Description: | Date Completed 12.05.2015 Date Revised 20.10.2016 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1939-165X |
| DOI: | 10.1111/vcp.12086 |