Differential induction of antioxidant stilbenoids in hairy roots of Vitis rotundifolia treated with methyl jasmonate and hydrogen peroxide

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 74(2014) vom: 01. Jan., Seite 50-69
1. Verfasser:	Nopo-Olazabal, Cesar (VerfasserIn)
Weitere Verfasser:	Condori, Jose, Nopo-Olazabal, Luis, Medina-Bolivar, Fabricio
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2,2-diphenyl-1-picrylhydrazyl 2,2′-azinobis[3-ethylbenzthiazoline sulfonic acid] 2,6 dimethyl-β-cyclodextrin ABTS Antioxidant BDS CHS mehr... CTAB DIMEB DPPH DW Dunstan and Short medium Elicitation EtOAc FW H(2)O(2) HPLC Hairy root JA MIQE MS MSV MeJA Minimum Information for Publication of Quantitative Real-Time PCR Experiments Murashige and Skoog medium Muscadine grape NF PAL RNA quality indicator ROS RQI RS Rt SD Stilbene synthase Stilbenoid TAC TEAC Trolox equivalent antioxidant capacity V Vitis rotundifolia cetyltrimethylammonium bromide chalcone synthase dry weight ethyl acetate fresh weight high performance liquid chromatography hydrogen peroxide jasmonic acid methyl jasmonate modified Murashige and Skoog medium normalization factor pairwise variation phenylalanine ammonia-lyase qPCR quantitative polymerase chain reaction reactive oxygen species resveratrol synthase retention time specific growth rate standard deviation total antioxidant capacity μ Acetates Antioxidants Benzothiazoles Biphenyl Compounds Culture Media Cyclopentanes Oxylipins Picrates RNA, Plant Stilbenes Sulfonic Acids 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid 28752-68-3 900N171A0F Hydrogen Peroxide BBX060AN9V 1,1-diphenyl-2-picrylhydrazyl DFD3H4VGDH

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier Masson SAS. All rights reserved. Stilbenoids are polyphenolic phytoalexins that exhibit potential health applications in humans. Hairy root cultures of muscadine grape (Vitis rotundifolia Michx.) were used to study the biochemical and molecular regulation of stilbenoid biosynthesis upon treatment with 100 μM methyl jasmonate (MeJA) or 10 mM hydrogen peroxide (H2O2) over a 96-h period. Resveratrol, piceid, and ε-viniferin were identified in higher concentrations in the tissue whereas resveratrol was the most abundant stilbenoid in the medium under either treatment. An earlier increase in resveratrol accumulation was observed for the MeJA-treated group showing a maximum at 12 h in the tissue and 18 h in the medium. Furthermore, the antioxidant capacity of extracts from the tissue and medium was determined by the 2,2'-azinobis[3-ethylbenzthiazoline sulfonic acid] (ABTS) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays showing correlation with the stilbenoid content. Fourteen candidate reference genes for qPCR were tested under the described experimental conditions and resulted in the selection of 5 reference genes. Quantitative analyses of transcripts for phenylalanine ammonia-lyase (PAL), resveratrol synthase (RS), and two stilbene synthases (STS and STS2) showed the highest RNA level induction at 3 h for both treatments with a higher induction for the MeJA treatment. In contrast, the flavonoid-related chalcone synthase (CHS) transcripts showed induction and a decrease in expression for MeJA and H2O2 treatments, respectively. The observed responses could be related to an oxidative burst triggered by the exposure to abiotic stressor compounds with signaling function such as MeJA and H2O2 which have been previously related to the synthesis of secondary metabolites
Beschreibung:	Date Completed 22.09.2014 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.10.035