|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM232500282 |
| 003 |
DE-627 |
| 005 |
20231224093122.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/ert344
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0775.xml
|
| 035 |
|
|
|a (DE-627)NLM232500282
|
| 035 |
|
|
|a (NLM)24215076
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Ma, Qiaoli
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 26.09.2014
|
| 500 |
|
|
|a Date Revised 21.10.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Auxin-like 2,4-dichlorophenoxyacetic acid (2,4-D), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. However, no other substitutes for 2,4-D are available to the post-harvest industry. Insights into the molecular mechanism underlying the effects of 2,4-D on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. This study comprehensively analysed changes in the peel of Olinda Valencia orange [Citrus sinensis (L.) Osbeck] induced by 500 ppm 2,4-D using 'omic'-driven approaches. Transcriptional profiling revealed that transcriptional factor (mainly AP2/ERF, WRKY, and NAC family members), transport, and hormone metabolism genes were over-represented and up-regulated within 24h post-treatment (HPT). Stress defence genes were up-regulated, while cell wall metabolism genes were down-regulated after 48 HPT. However, secondary metabolism genes, especially phenylpropanoid and lignin biosynthesis-related genes, were over-represented at all the time points. Comparative proteomic analysis indicated that the expression of proteins implicated in stress responses (25%), hormone metabolism, and signal transduction (12%) significantly accumulated at the post-transcriptional level. Hormone levels detected by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) showed that abscisic acid, salicylic acid, and 2,4-D significantly increased, while ethylene production (detected by gas chromatography) decreased after 2,4-D treatment. In addition, lignin and water content in the fruit peel also increased and the epicuticle wax ultrastructure was modified. In conclusion, 2,4-D retarded fruit senescence by altering the levels of many endogenous hormones and by improving stress defence capabilities by up-regulating defence-related genes and proteins
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a 2,4-dichlorophenoxy acetic acid (2,4-D)
|
| 650 |
|
4 |
|a ABA
|
| 650 |
|
4 |
|a auxin
|
| 650 |
|
4 |
|a citrus fruit
|
| 650 |
|
4 |
|a ethylene
|
| 650 |
|
4 |
|a fruit quality preservation
|
| 650 |
|
4 |
|a fruit senescence
|
| 650 |
|
4 |
|a plant hormones
|
| 650 |
|
4 |
|a stress defence.
|
| 650 |
|
7 |
|a Ethylenes
|2 NLM
|
| 650 |
|
7 |
|a Indoleacetic Acids
|2 NLM
|
| 650 |
|
7 |
|a Plant Growth Regulators
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Water
|2 NLM
|
| 650 |
|
7 |
|a 059QF0KO0R
|2 NLM
|
| 650 |
|
7 |
|a 2,4-Dichlorophenoxyacetic Acid
|2 NLM
|
| 650 |
|
7 |
|a 2577AQ9262
|2 NLM
|
| 650 |
|
7 |
|a Abscisic Acid
|2 NLM
|
| 650 |
|
7 |
|a 72S9A8J5GW
|2 NLM
|
| 650 |
|
7 |
|a Lignin
|2 NLM
|
| 650 |
|
7 |
|a 9005-53-2
|2 NLM
|
| 650 |
|
7 |
|a ethylene
|2 NLM
|
| 650 |
|
7 |
|a 91GW059KN7
|2 NLM
|
| 650 |
|
7 |
|a Salicylic Acid
|2 NLM
|
| 650 |
|
7 |
|a O414PZ4LPZ
|2 NLM
|
| 700 |
1 |
|
|a Ding, Yuduan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chang, Jiwei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sun, Xiaohua
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhang, Li
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wei, Qingjiang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Cheng, Yunjiang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chen, Lingling
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xu, Juan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Deng, Xiuxin
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 1 vom: 25. Jan., Seite 61-74
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:65
|g year:2014
|g number:1
|g day:25
|g month:01
|g pages:61-74
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/ert344
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 65
|j 2014
|e 1
|b 25
|c 01
|h 61-74
|