JAXA protein crystallization in space ongoing improvements for growing high-quality crystals

JAXA protein crystallization in space : ongoing improvements for growing high-quality crystals

The Japan Aerospace Exploration Agency (JAXA) started a high-quality protein crystal growth project, now called JAXA PCG, on the International Space Station (ISS) in 2002. Using the counter-diffusion technique, 14 sessions of experiments have been performed as of 2012 with 580 proteins crystallized...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 20(2013), Pt 6 vom: 07. Nov., Seite 968-73
1. Verfasser: Takahashi, Sachiko (VerfasserIn)
Weitere Verfasser: Ohta, Kazunori, Furubayashi, Naoki, Yan, Bin, Koga, Misako, Wada, Yoshio, Yamada, Mitsugu, Inaka, Koji, Tanaka, Hiroaki, Miyoshi, Hiroshi, Kobayashi, Tomoyuki, Kamigaichi, Shigeki
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article JAXA Japan Experiment Module `Kibo' counter-diffusion impurity depletion zone microgravity protein crystal protein depletion zone Proteins
Beschreibung
Zusammenfassung:The Japan Aerospace Exploration Agency (JAXA) started a high-quality protein crystal growth project, now called JAXA PCG, on the International Space Station (ISS) in 2002. Using the counter-diffusion technique, 14 sessions of experiments have been performed as of 2012 with 580 proteins crystallized in total. Over the course of these experiments, a user-friendly interface framework for high accessibility has been constructed and crystallization techniques improved; devices to maximize the use of the microgravity environment have been designed, resulting in some high-resolution crystal growth. If crystallization conditions were carefully fixed in ground-based experiments, high-quality protein crystals grew in microgravity in many experiments on the ISS, especially when a highly homogeneous protein sample and a viscous crystallization solution were employed. In this article, the current status of JAXA PCG is discussed, and a rational approach to high-quality protein crystal growth in microgravity based on numerical analyses is explained
Beschreibung:Date Completed 22.05.2014
Date Revised 21.10.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1600-5775
DOI:10.1107/S0909049513021596