Phase-diagram-guided method for growth of a large crystal of glycoside hydrolase family 45 inverting cellulase suitable for neutron structural analysis
Neutron protein crystallography (NPC) is a powerful tool for determining the hydrogen position and water orientation in proteins, but a much larger protein crystal is needed for NPC than for X-ray crystallography, and thus crystal preparation is a bottleneck. To obtain large protein crystals, it is...
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Bibliographische Detailangaben
| Veröffentlicht in: | Journal of synchrotron radiation. - 1994. - 20(2013), Pt 6 vom: 07. Nov., Seite 859-63
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| 1. Verfasser: |
Nakamura, Akihiko
(VerfasserIn) |
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| Weitere Verfasser: |
Ishida, Takuya,
Fushinobu, Shinya,
Kusaka, Katsuhiro,
Tanaka, Ichiro,
Inaka, Koji,
Higuchi, Yoshiki,
Masaki, Mika,
Ohta, Kazunori,
Kaneko, Satoshi,
Niimura, Nobuo,
Igarashi, Kiyohiko,
Samajima, Masahiro |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | Journal of synchrotron radiation
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
cellulase
crystallization phase diagram
neutron protein crystallography
Glycoside Hydrolases
EC 3.2.1.-
Cellulase
EC 3.2.1.4 |
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