Peroxidation due to cryoprotectant treatment is a vital factor for cell survival in Arabidopsis cryopreservation
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
| Veröffentlicht in: | Plant science : an international journal of experimental plant biology. - 1985. - 212(2013) vom: 07. Nov., Seite 37-47 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2013
|
| Zugriff auf das übergeordnete Werk: | Plant science : an international journal of experimental plant biology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't AFLPa Arabidopsis thaliana BLAST Cryopreservation EST FDA LN MDA mehr... |
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM231405626 | ||
| 003 | DE-627 | ||
| 005 | 20250216002744.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.plantsci.2013.07.011 |2 doi | |
| 028 | 5 | 2 | |a pubmed25n0771.xml |
| 035 | |a (DE-627)NLM231405626 | ||
| 035 | |a (NLM)24094052 | ||
| 035 | |a (PII)S0168-9452(13)00167-2 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ren, Li |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Peroxidation due to cryoprotectant treatment is a vital factor for cell survival in Arabidopsis cryopreservation |
| 264 | 1 | |c 2013 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 07.05.2014 | ||
| 500 | |a Date Revised 10.03.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a GENBANK: HO850201, HO850202, HO850203, HO850204, HO850205, HO850206, HO850207, HO850208, HO850209, HO850210, HO850211, HO850212, HO850213, HO850214, HO850215, HO850216, HO850217, HO850218, HO850219, HO850220, HO850221, HO850222, HO850223, HO850224, HO850225, HO850226, HO850227, HO850228, HO850229, HO850230, HO850231, HO850232, HO850233, HO850234, HO850235, HO850236, HO850237, HO850238, HO850239, HO850240, HO850241, HO850242, HO850243, HO850244, HO850245, HO850246, HO850247, HO850248, HO850249, HO850250, HO850251, HO850252, HO850253, HO850254, HO850255, HO850256, HO850257, HO850258, HO850259, HO850260, HO850261, HO850262, HO850263, HO850264, HO850265, HO850266, HO850267, HO850268, HO850269, HO850270, HO850271, HO850272, HO850273, HO850274, HO850275, HO850276, HO850277, HO850278, HO850279, HO850280, HO850281, HO850282, HO850283, HO850284, HO850285, HO850286, HO850287, HO850288, HO850289, HO850290, HO850291, HO850292, HO850293, HO850294, HO850295, HO850296, HO850297, HO850298, HO850299, HO850300, HO850301, HO850302, HO850303, HO850304, HO850305, HO850306, HO850307, HO850308, HO850309, HO850310, HO850311, HO850312, HO850313, HO850314, HO850315, HO850316, HO850317, HO850318, HO850319, HO850320, HO850321, HO850322, HO850323, HO850324, HO850325, HO850326, HO850327, HO850328, HO850329, HO850330, HO850331, HO850332, HO850333, HO850334, HO850335, HO850336, HO850337, HO850338, HO850339, HO850340, HO850341, HO850342, HO850343, HO850344, HO850345 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. | ||
| 520 | |a Cryopreservation can be a safe and cost-effective tool for the long-term storage of plant germplasm. In Arabidopsis, the ability to recover from cryogenic treatment was lost as growth progressed. Growth could be restored in 48-h seedlings, whereas 72-h seedlings died after cryogenic treatment. Why seedling age and survival are negatively correlated is an interesting issue. A comparative transcriptomics was performed to screen differentially expressed genes between 48- and 72-h seedlings after exposure to cryoprotectant. Among differentially expressed genes, oxidative stress response genes played important roles in cryoprotectant treatment, and peroxidation was a key factor related to cell survival. Seedlings underwent more peroxidation at 72-h than at 48-h. A comprehensive analysis indicated that peroxidation injured membrane systems leading to photophosphorylation and oxidative phosphorylation damage. Furthermore, the apoptosis-like events were found in cryogenic treatment of Arabidopsis seedlings. 48- and 72-h seedlings underwent different degrees of membrane lipid peroxidation during cryoprotectant treatment, and reducing the injury of oxidative stress was an important factor to successful cryopreservation. This study provided a novel insight of genetic regulatory mechanisms in cryopreservation, and established an excellent model to test and evaluate the effect of exogenous antioxidants and conventional cryoprotectants in plant cryopreservation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a AFLPa | |
| 650 | 4 | |a Arabidopsis thaliana | |
| 650 | 4 | |a BLAST | |
| 650 | 4 | |a Cryopreservation | |
| 650 | 4 | |a EST | |
| 650 | 4 | |a FDA | |
| 650 | 4 | |a LN | |
| 650 | 4 | |a MDA | |
| 650 | 4 | |a MS | |
| 650 | 4 | |a Murashige and Skoog [1] | |
| 650 | 4 | |a NCBI | |
| 650 | 4 | |a National Center for Biotechnology Information | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 4 | |a PVS | |
| 650 | 4 | |a Peroxidation | |
| 650 | 4 | |a ROS | |
| 650 | 4 | |a TBA | |
| 650 | 4 | |a TCA | |
| 650 | 4 | |a TDFs | |
| 650 | 4 | |a basic local alignment search tool | |
| 650 | 4 | |a cDNA-AFLP | |
| 650 | 4 | |a expressed sequence tag | |
| 650 | 4 | |a fluorescein diacetate | |
| 650 | 4 | |a liquid nitrogen | |
| 650 | 4 | |a malondialdehyde | |
| 650 | 4 | |a mplified fragment length polymorphism | |
| 650 | 4 | |a plant vitrification solution | |
| 650 | 4 | |a qRT-PCR | |
| 650 | 4 | |a quantitative real time-PCR | |
| 650 | 4 | |a reactive oxygen species | |
| 650 | 4 | |a thiobarbituric acid | |
| 650 | 4 | |a transcript-derived fragments | |
| 650 | 4 | |a trichloroacetic acid | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Cryoprotective Agents |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 700 | 1 | |a Zhang, Di |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Xiang-Ning |e verfasserin |4 aut | |
| 700 | 1 | |a Gai, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wei-Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Reed, Barbara M |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Xiao-Hui |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Plant science : an international journal of experimental plant biology |d 1985 |g 212(2013) vom: 07. Nov., Seite 37-47 |w (DE-627)NLM098174193 |x 1873-2259 |7 nnns |
| 773 | 1 | 8 | |g volume:212 |g year:2013 |g day:07 |g month:11 |g pages:37-47 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.plantsci.2013.07.011 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 912 | |a GBV_ILN_350 | ||
| 951 | |a AR | ||
| 952 | |d 212 |j 2013 |b 07 |c 11 |h 37-47 | ||