Peroxidation due to cryoprotectant treatment is a vital factor for cell survival in Arabidopsis cryopreservation
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
| Veröffentlicht in: | Plant science : an international journal of experimental plant biology. - 1985. - 212(2013) vom: 07. Nov., Seite 37-47 |
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| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | Plant science : an international journal of experimental plant biology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't AFLPa Arabidopsis thaliana BLAST Cryopreservation EST FDA LN MDA mehr... |
| Zusammenfassung: | Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. Cryopreservation can be a safe and cost-effective tool for the long-term storage of plant germplasm. In Arabidopsis, the ability to recover from cryogenic treatment was lost as growth progressed. Growth could be restored in 48-h seedlings, whereas 72-h seedlings died after cryogenic treatment. Why seedling age and survival are negatively correlated is an interesting issue. A comparative transcriptomics was performed to screen differentially expressed genes between 48- and 72-h seedlings after exposure to cryoprotectant. Among differentially expressed genes, oxidative stress response genes played important roles in cryoprotectant treatment, and peroxidation was a key factor related to cell survival. Seedlings underwent more peroxidation at 72-h than at 48-h. A comprehensive analysis indicated that peroxidation injured membrane systems leading to photophosphorylation and oxidative phosphorylation damage. Furthermore, the apoptosis-like events were found in cryogenic treatment of Arabidopsis seedlings. 48- and 72-h seedlings underwent different degrees of membrane lipid peroxidation during cryoprotectant treatment, and reducing the injury of oxidative stress was an important factor to successful cryopreservation. This study provided a novel insight of genetic regulatory mechanisms in cryopreservation, and established an excellent model to test and evaluate the effect of exogenous antioxidants and conventional cryoprotectants in plant cryopreservation |
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| Beschreibung: | Date Completed 07.05.2014 Date Revised 10.03.2022 published: Print-Electronic GENBANK: HO850201, HO850202, HO850203, HO850204, HO850205, HO850206, HO850207, HO850208, HO850209, HO850210, HO850211, HO850212, HO850213, HO850214, HO850215, HO850216, HO850217, HO850218, HO850219, HO850220, HO850221, HO850222, HO850223, HO850224, HO850225, HO850226, HO850227, HO850228, HO850229, HO850230, HO850231, HO850232, HO850233, HO850234, HO850235, HO850236, HO850237, HO850238, HO850239, HO850240, HO850241, HO850242, HO850243, HO850244, HO850245, HO850246, HO850247, HO850248, HO850249, HO850250, HO850251, HO850252, HO850253, HO850254, HO850255, HO850256, HO850257, HO850258, HO850259, HO850260, HO850261, HO850262, HO850263, HO850264, HO850265, HO850266, HO850267, HO850268, HO850269, HO850270, HO850271, HO850272, HO850273, HO850274, HO850275, HO850276, HO850277, HO850278, HO850279, HO850280, HO850281, HO850282, HO850283, HO850284, HO850285, HO850286, HO850287, HO850288, HO850289, HO850290, HO850291, HO850292, HO850293, HO850294, HO850295, HO850296, HO850297, HO850298, HO850299, HO850300, HO850301, HO850302, HO850303, HO850304, HO850305, HO850306, HO850307, HO850308, HO850309, HO850310, HO850311, HO850312, HO850313, HO850314, HO850315, HO850316, HO850317, HO850318, HO850319, HO850320, HO850321, HO850322, HO850323, HO850324, HO850325, HO850326, HO850327, HO850328, HO850329, HO850330, HO850331, HO850332, HO850333, HO850334, HO850335, HO850336, HO850337, HO850338, HO850339, HO850340, HO850341, HO850342, HO850343, HO850344, HO850345 Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2013.07.011 |