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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1021/la402139g
|2 doi
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|a eng
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|a Kusolkamabot, Keerati
|e verfasserin
|4 aut
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|a Poly(N-isopropylacrylamide)-stabilized gold nanoparticles in combination with tricationic branched phenylene-ethynylene fluorophore for protein identification
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 29.04.2014
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|a Date Revised 16.11.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Gold nanoparticles stabilized by thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAM-AuNPs) were prepared by surface grafting of thiol-terminated PNIPAM onto citrate-stabilized AuNPs. The color change of the PNIPAM-AuNPs solution from red to blue-purple without precipitation when the solution was heated to 40 °C, above the lower critical solution temperature (LCST) of PNIPAM, indicated the thermoresponsive property of the synthesized AuNPs. PNIPAM-AuNPs were used to detect proteins by chemical nose approach based on fluorescence quenching of fluorophore by AuNPs. An array-based sensing platform for detection of six proteins, namely bovine serum albumin, lysozyme, fibrinogen, concanavalin A, hemoglobin, holo-transferrin human can be successfully developed from the PNIPAM-AuNPs having different molecular weights (4 and 8 kDa) and conformation (varied heat treatment from 25 to 40 °C) in combination with a tricationic branched phenylene-ethynylene fluorophore. From principal component analysis (PCA) followed by linear discriminant analysis (LDA), 100% accuracy of protein classification using a leave-one-out (LOO) approach can be achieved by using only two types of PNIPAM-AuNPs
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Acrylic Resins
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|a Alkynes
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|a Cations
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|a Hemoglobins
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|a Transferrin
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|a Concanavalin A
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|a Serum Albumin, Bovine
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|a 27432CM55Q
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|a Gold
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|a 7440-57-5
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|a Fibrinogen
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|a Muramidase
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|a EC 3.2.1.17
|2 NLM
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|a Sae-ung, Pornpen
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|4 aut
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|a Niamnont, Nakorn
|e verfasserin
|4 aut
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|a Wongravee, Kanet
|e verfasserin
|4 aut
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|a Sukwattanasinitt, Mongkol
|e verfasserin
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|a Hoven, Voravee P
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 29(2013), 39 vom: 01. Okt., Seite 12317-27
|w (DE-627)NLM098181009
|x 1520-5827
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| 773 |
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|g volume:29
|g year:2013
|g number:39
|g day:01
|g month:10
|g pages:12317-27
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|u http://dx.doi.org/10.1021/la402139g
|3 Volltext
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