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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2013.06.015
|2 doi
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|a pubmed24n0764.xml
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|a (DE-627)NLM229212867
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|a (NLM)23860230
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|a (PII)S0981-9428(13)00246-5
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Chen, Qi
|e verfasserin
|4 aut
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| 245 |
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|a Up-regulation and interaction of the plasma membrane H(+)-ATPase and the 14-3-3 protein are involved in the regulation of citrate exudation from the broad bean (Vicia faba L.) under Al stress
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.02.2014
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Masson SAS. All rights reserved.
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|a Our previous study showed that citrate excretion coupled with a concomitant release of protons was involved in aluminum (Al) resistance in the broad bean. Furthermore, genes encoding plasma membrane (PM) H(+)-ATPase (vha2) and the 14-3-3 protein (vf14-3-3b) were up-regulated by Al in Al-resistant (YD) broad bean roots. In this study, the roles of PM H(+)-ATPase (E.C. 3.6.3.6) and the 14-3-3 protein in the regulation of citrate secretion were further investigated in Al-resistant (YD) and Al-sensitive (AD) broad bean cultivars under Al stress. The results showed that greater citrate exudation was positively correlated with higher activities of PM H(+)-ATPase in roots of YD than AD. Real-time RT-PCR analysis revealed that vha2 was clearly up-regulated by Al in YD but not in AD roots, whereas the transcription levels of vf14-3-3b were elevated in a time-dependent manner in both YD and AD roots. Immunoprecipitation and Western analysis suggested that phosphorylation and interaction with the vf14-3-3b protein of the VHA2 were enhanced in YD roots but not in AD roots with increasing Al treatment time. Fusicoccin or adenosine 5'-monophosphate increased or decreased the interaction between the phosphorylated VHA2 and the vf14-3-3b protein, followed by an enhancement or reduction of the PM H(+)-ATPase activity and citrate exudation in both cultivars under Al stress conditions, respectively. Taken together, these results suggested that Al enhanced the expression and interaction of the PM H(+)-ATPase and the 14-3-3 protein, which thereby led to higher activity of the PM H(+)-ATPase and more citrate exudation from YD plants
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 1,3-bis-[tris(hy- droxymethyl)-methylamino]-propane
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|a 14-3-3 Protein
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|a 5’-AMP
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|a AD
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|a Al
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|a Al stress
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|a BTP
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|a Broad bean
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|a Citrate exudation
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|a DDT
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|a FC
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|a OA
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| 650 |
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|a PM
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|a PMSF
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| 650 |
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|a Plasma membrane H(+)-ATPase
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|a VHA2p
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|a Vicia faba 14-3-3-like protein b
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| 650 |
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|a Vicia faba plasma membrane H(+)-ATPase
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|a Vicia faba. L cv. Anhui Candou
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|a Vicia faba. L cv. Yunnan Candou 8363
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|a YD
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|a adenosine 5′-monophosphate
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|a aluminum
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|a dithiothreitol
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|a fusicoccin
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|a organic acid
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|a phenylmethylsulfonyl fluoride
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| 650 |
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|a phosphorylated VHA2 at Thr-951 in C terminus
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|a plasma membrane
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|a vf14-3-3b
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|a vha2
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|a 14-3-3 Proteins
|2 NLM
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|a Glycosides
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a fusicoccin
|2 NLM
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|a 20108-30-9
|2 NLM
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| 650 |
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|a Citric Acid
|2 NLM
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| 650 |
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|a 2968PHW8QP
|2 NLM
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| 650 |
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|a Adenosine Monophosphate
|2 NLM
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| 650 |
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|a 415SHH325A
|2 NLM
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| 650 |
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|a Aluminum
|2 NLM
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| 650 |
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|a CPD4NFA903
|2 NLM
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| 650 |
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|a Proton-Translocating ATPases
|2 NLM
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| 650 |
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|a EC 3.6.3.14
|2 NLM
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| 700 |
1 |
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|a Guo, Chuan-Long
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Ping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Xuan-Qin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Kong-Huan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Kui-Zhi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Yong-Xiong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Li-Mei
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 70(2013) vom: 15. Sept., Seite 504-11
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:70
|g year:2013
|g day:15
|g month:09
|g pages:504-11
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|u http://dx.doi.org/10.1016/j.plaphy.2013.06.015
|3 Volltext
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|d 70
|j 2013
|b 15
|c 09
|h 504-11
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