Up-regulation and interaction of the plasma membrane H(+)-ATPase and the 14-3-3 protein are involved in the regulation of citrate exudation from the broad bean (Vicia faba L.) under Al stress

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 70(2013) vom: 15. Sept., Seite 504-11
1. Verfasser:	Chen, Qi (VerfasserIn)
Weitere Verfasser:	Guo, Chuan-Long, Wang, Ping, Chen, Xuan-Qin, Wu, Kong-Huan, Li, Kui-Zhi, Yu, Yong-Xiong, Chen, Li-Mei
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't 1,3-bis-[tris(hy- droxymethyl)-methylamino]-propane 14-3-3 Protein 5’-AMP AD Al Al stress BTP Broad bean mehr... Citrate exudation DDT FC OA PM PMSF Plasma membrane H(+)-ATPase VHA2p Vicia faba 14-3-3-like protein b Vicia faba plasma membrane H(+)-ATPase Vicia faba. L cv. Anhui Candou Vicia faba. L cv. Yunnan Candou 8363 YD adenosine 5′-monophosphate aluminum dithiothreitol fusicoccin organic acid phenylmethylsulfonyl fluoride phosphorylated VHA2 at Thr-951 in C terminus plasma membrane vf14-3-3b vha2 14-3-3 Proteins Glycosides Plant Proteins 20108-30-9 Citric Acid 2968PHW8QP Adenosine Monophosphate 415SHH325A Aluminum CPD4NFA903 Proton-Translocating ATPases EC 3.6.3.14

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier Masson SAS. All rights reserved. Our previous study showed that citrate excretion coupled with a concomitant release of protons was involved in aluminum (Al) resistance in the broad bean. Furthermore, genes encoding plasma membrane (PM) H(+)-ATPase (vha2) and the 14-3-3 protein (vf14-3-3b) were up-regulated by Al in Al-resistant (YD) broad bean roots. In this study, the roles of PM H(+)-ATPase (E.C. 3.6.3.6) and the 14-3-3 protein in the regulation of citrate secretion were further investigated in Al-resistant (YD) and Al-sensitive (AD) broad bean cultivars under Al stress. The results showed that greater citrate exudation was positively correlated with higher activities of PM H(+)-ATPase in roots of YD than AD. Real-time RT-PCR analysis revealed that vha2 was clearly up-regulated by Al in YD but not in AD roots, whereas the transcription levels of vf14-3-3b were elevated in a time-dependent manner in both YD and AD roots. Immunoprecipitation and Western analysis suggested that phosphorylation and interaction with the vf14-3-3b protein of the VHA2 were enhanced in YD roots but not in AD roots with increasing Al treatment time. Fusicoccin or adenosine 5'-monophosphate increased or decreased the interaction between the phosphorylated VHA2 and the vf14-3-3b protein, followed by an enhancement or reduction of the PM H(+)-ATPase activity and citrate exudation in both cultivars under Al stress conditions, respectively. Taken together, these results suggested that Al enhanced the expression and interaction of the PM H(+)-ATPase and the 14-3-3 protein, which thereby led to higher activity of the PM H(+)-ATPase and more citrate exudation from YD plants
Beschreibung:	Date Completed 13.02.2014 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.06.015