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231224s2013 xx |||||o 00| ||eng c |
| 024 |
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|a 10.1111/nph.12369
|2 doi
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|a pubmed24n0761.xml
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|a (DE-627)NLM228547636
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|a (NLM)23790109
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Takagi, Hiroki
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a MutMap-Gap
|b whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 15.04.2014
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| 500 |
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|a Date Revised 10.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
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|a Next-generation sequencing allows the identification of mutations responsible for mutant phenotypes by whole-genome resequencing and alignment to a reference genome. However, when the resequenced cultivar/line displays significant structural variation from the reference genome, mutations in the genome regions missing from the reference (gaps) cannot be identified by simple alignment. Here we report on a method called 'MutMap-Gap', which involves delineating a candidate region harboring a mutation of interest using the recently reported MutMap method, followed by de novo assembly, alignment, and identification of the mutation within genome gaps. We applied MutMap-Gap to isolate the blast resistant gene Pii from the rice cv Hitomebore using mutant lines that have lost Pii function. MutMap-Gap should prove useful for cloning genes that exhibit significant structural variations such as disease resistance genes of the nucleotide-binding site-leucine rich repeat (NBS-LRR) class
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a MutMap
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| 650 |
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4 |
|a R-gene
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| 650 |
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4 |
|a de novo assembly
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| 650 |
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4 |
|a next-generation sequencing
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| 650 |
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4 |
|a rice
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| 650 |
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4 |
|a whole-genome sequencing
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| 650 |
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7 |
|a PII Nitrogen Regulatory Proteins
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Uemura, Aiko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yaegashi, Hiroki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tamiru, Muluneh
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Abe, Akira
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mitsuoka, Chikako
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Utsushi, Hiroe
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Natsume, Satoshi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kanzaki, Hiroyuki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Matsumura, Hideo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Saitoh, Hiromasa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yoshida, Kentaro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cano, Liliana M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kamoun, Sophien
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Terauchi, Ryohei
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 200(2013), 1 vom: 19. Okt., Seite 276-283
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
8 |
|g volume:200
|g year:2013
|g number:1
|g day:19
|g month:10
|g pages:276-283
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/nph.12369
|3 Volltext
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|a AR
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| 952 |
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|d 200
|j 2013
|e 1
|b 19
|c 10
|h 276-283
|