MutMap-Gap whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii

MutMap-Gap : whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii

© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 200(2013), 1 vom: 19. Okt., Seite 276-283
1. Verfasser: Takagi, Hiroki (VerfasserIn)
Weitere Verfasser: Uemura, Aiko, Yaegashi, Hiroki, Tamiru, Muluneh, Abe, Akira, Mitsuoka, Chikako, Utsushi, Hiroe, Natsume, Satoshi, Kanzaki, Hiroyuki, Matsumura, Hideo, Saitoh, Hiromasa, Yoshida, Kentaro, Cano, Liliana M, Kamoun, Sophien, Terauchi, Ryohei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't MutMap R-gene de novo assembly next-generation sequencing rice whole-genome sequencing PII Nitrogen Regulatory Proteins Plant Proteins
Beschreibung
Zusammenfassung:© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
Next-generation sequencing allows the identification of mutations responsible for mutant phenotypes by whole-genome resequencing and alignment to a reference genome. However, when the resequenced cultivar/line displays significant structural variation from the reference genome, mutations in the genome regions missing from the reference (gaps) cannot be identified by simple alignment. Here we report on a method called 'MutMap-Gap', which involves delineating a candidate region harboring a mutation of interest using the recently reported MutMap method, followed by de novo assembly, alignment, and identification of the mutation within genome gaps. We applied MutMap-Gap to isolate the blast resistant gene Pii from the rice cv Hitomebore using mutant lines that have lost Pii function. MutMap-Gap should prove useful for cloning genes that exhibit significant structural variations such as disease resistance genes of the nucleotide-binding site-leucine rich repeat (NBS-LRR) class
Beschreibung:Date Completed 15.04.2014
Date Revised 10.04.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.12369