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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2013.05.031
|2 doi
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|a pubmed24n1222.xml
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|a (DE-627)NLM228386594
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|a (NLM)23771036
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|a (PII)S0981-9428(13)00205-2
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Gharechahi, Javad
|e verfasserin
|4 aut
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|a An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 13.02.2014
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Masson SAS. All rights reserved.
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|a Jasmonate and its methyl derivative, methyl jasmonate (MeJA), are naturally occurring compounds that mediate several plant physiological processes in response to pathogen attack, wounding, and ozone. Exogenous application of jasmonates triggers defense responses that resemble those initiated by pathogen infection and also modulates the production of certain secondary metabolites in a variety of plant species. In this study, we treated the hairy root cultures of Silybum marianum L. with 100 μM MeJA and then measured the content of Silymarin (SLM). We observed that the SLM content increased significantly after 48 h of MeJA treatment and remained constant for 120 h. However, MeJA treatment caused a significant growth reduction after 96 h incubation. The activity of lipoxygenase as a key enzyme in the jasmonate biosynthesis pathway and anti-oxidative enzymes; peroxidase and ascorbate peroxidase was also significantly increased after MeJA treatment. To elucidate the global effect of jasmonate on gene expression of S. marianum, we employed high resolution two-dimensional gel electrophoresis coupled with tandem mass spectrometry. Out of 670 reproducibly detected protein spots which were analyzed on each given gel, 32 spots were up- or down regulated upon MeJA treatment. Of them, ten proteins such as ER binding protein, glutamine synthetase, pathogenesis-related protein, caffeoyl CoA O-methyltransferase, and profilin-1 could be identified by mass spectrometry analysis. The possible implications of the identified proteins on physiological outcome of MeJA application in S. marianum hairy root culture will be discussed
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 2-DE
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|a APX
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|a CBB
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|a FAME
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|a GC
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|a HPLC
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|a Hairy root culture
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|a IEF
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|a ISB
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|a IgG
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|a JA
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|a LOX
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|a MALDI-TOF/TOF
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|a MS
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|a Mass spectrometry
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|a MeJA
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|a Methyl jasmonate (MeJA)
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|a PMF
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|a POD
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|a SB
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|a SC
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|a SD
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|a SDS-PAGE
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|a SLM
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|a Silymarin (SLM)
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|a TXF
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|a Two-dimensional gel electrophoresis
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|a ascorbate peroxidase
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|a coomassie brilliant blue
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|a fatty acid methyl esters
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|a gas chromatography
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|a high-performance liquid chromatography
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|a immune globulin G
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|a isoelectric focusing
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|a isosilybinin
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|a jasmonic acid
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|a lipoxygenase
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|a mass spectrometry
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|a matrix assisted laser desorption ionization-time of flight–time of flight
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|a methyl jasmonate
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|a peptide mass finger printing
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|a peroxidase
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|a silybinin
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|a silycristin
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4 |
|a silydianin
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4 |
|a silymarin
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4 |
|a sodium dodecyl sulfate-poly acrylamide gel electrophoresis
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4 |
|a taxifolin
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|a two-dimensional gel electrophoresis
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|a Acetates
|2 NLM
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|a Antioxidants
|2 NLM
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|a Cyclopentanes
|2 NLM
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|a Oxylipins
|2 NLM
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|a Plant Growth Regulators
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Proteome
|2 NLM
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|a Silymarin
|2 NLM
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|a methyl jasmonate
|2 NLM
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|a 900N171A0F
|2 NLM
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|a Ascorbate Peroxidases
|2 NLM
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|a EC 1.11.1.11
|2 NLM
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|a Peroxidase
|2 NLM
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|a EC 1.11.1.7
|2 NLM
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|a Lipoxygenase
|2 NLM
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| 650 |
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|a EC 1.13.11.12
|2 NLM
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1 |
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|a Khalili, Masumeh
|e verfasserin
|4 aut
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1 |
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|a Hasanloo, Tahereh
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Salekdeh, Ghasem Hosseini
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 70(2013) vom: 26. Sept., Seite 115-22
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:70
|g year:2013
|g day:26
|g month:09
|g pages:115-22
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|u http://dx.doi.org/10.1016/j.plaphy.2013.05.031
|3 Volltext
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|d 70
|j 2013
|b 26
|c 09
|h 115-22
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