An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots

An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 70(2013) vom: 26. Sept., Seite 115-22
1. Verfasser: Gharechahi, Javad (VerfasserIn)
Weitere Verfasser: Khalili, Masumeh, Hasanloo, Tahereh, Salekdeh, Ghasem Hosseini
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 2-DE APX CBB FAME GC HPLC Hairy root culture IEF mehr... ISB IgG JA LOX MALDI-TOF/TOF MS Mass spectrometry MeJA Methyl jasmonate (MeJA) PMF POD SB SC SD SDS-PAGE SLM Silymarin (SLM) TXF Two-dimensional gel electrophoresis ascorbate peroxidase coomassie brilliant blue fatty acid methyl esters gas chromatography high-performance liquid chromatography immune globulin G isoelectric focusing isosilybinin jasmonic acid lipoxygenase mass spectrometry matrix assisted laser desorption ionization-time of flight–time of flight methyl jasmonate peptide mass finger printing peroxidase silybinin silycristin silydianin silymarin sodium dodecyl sulfate-poly acrylamide gel electrophoresis taxifolin two-dimensional gel electrophoresis Acetates Antioxidants Cyclopentanes Oxylipins Plant Growth Regulators Plant Proteins Proteome Silymarin 900N171A0F Ascorbate Peroxidases EC 1.11.1.11 Peroxidase EC 1.11.1.7 Lipoxygenase EC 1.13.11.12
Beschreibung
Zusammenfassung:Copyright © 2013 Elsevier Masson SAS. All rights reserved.
Jasmonate and its methyl derivative, methyl jasmonate (MeJA), are naturally occurring compounds that mediate several plant physiological processes in response to pathogen attack, wounding, and ozone. Exogenous application of jasmonates triggers defense responses that resemble those initiated by pathogen infection and also modulates the production of certain secondary metabolites in a variety of plant species. In this study, we treated the hairy root cultures of Silybum marianum L. with 100 μM MeJA and then measured the content of Silymarin (SLM). We observed that the SLM content increased significantly after 48 h of MeJA treatment and remained constant for 120 h. However, MeJA treatment caused a significant growth reduction after 96 h incubation. The activity of lipoxygenase as a key enzyme in the jasmonate biosynthesis pathway and anti-oxidative enzymes; peroxidase and ascorbate peroxidase was also significantly increased after MeJA treatment. To elucidate the global effect of jasmonate on gene expression of S. marianum, we employed high resolution two-dimensional gel electrophoresis coupled with tandem mass spectrometry. Out of 670 reproducibly detected protein spots which were analyzed on each given gel, 32 spots were up- or down regulated upon MeJA treatment. Of them, ten proteins such as ER binding protein, glutamine synthetase, pathogenesis-related protein, caffeoyl CoA O-methyltransferase, and profilin-1 could be identified by mass spectrometry analysis. The possible implications of the identified proteins on physiological outcome of MeJA application in S. marianum hairy root culture will be discussed
Beschreibung:Date Completed 13.02.2014
Date Revised 13.12.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2013.05.031