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024 7 |a 10.1016/j.jplph.2013.04.008  |2 doi 
028 5 2 |a pubmed24n0760.xml 
035 |a (DE-627)NLM228165938 
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041 |a eng 
100 1 |a Aremu, Adeyemi O  |e verfasserin  |4 aut 
245 1 0 |a Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins 
264 1 |c 2013 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 13.03.2014 
500 |a Date Revised 30.09.2020 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2013 Elsevier GmbH. All rights reserved. 
520 |a Merwilla plumbea (Lindl.) Speta is an important medicinal plant widely used in traditional medicine. We evaluated the effect of five cytokinins [benzyladenine (BA), 2-isopentenyladenine (2iP), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxy-9-tetrahydropyran-2-yl-topolin (MemTTHP)] on the level of phenolic acids and antioxidant activity of M. plumbea during the tissue culture and acclimatization stages. Two cytokinins (mT and mTR) significantly improved the antioxidant activity of tissue culture plantlets while the control plantlets were better after acclimatization. Using UPLC-MS/MS, the levels of hydroxybenzoic and hydroxycinnamic acid derivatives (phenolic acids) varied significantly during tissue culture and acclimatization, depending on the cytokinin and plant part analyzed. Vanillic acid (24.9 μg g⁻¹) detected in underground parts of tissue culture plants supplemented with BA was the most abundant phenolic acid detected. The current findings indicate that the phytochemicals together with the bioactivity during in vitro propagation of M. plumbea is influenced by the cytokinin type used and the stage of plant material collection 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a 2,2′-azobis(2-methylpropionamidine) dihydrochloride 
650 4 |a 2-isopentenyladenine 
650 4 |a 2iP 
650 4 |a AAPH 
650 4 |a ANOVA 
650 4 |a BA 
650 4 |a CK 
650 4 |a Conservation 
650 4 |a DW 
650 4 |a MS 
650 4 |a Medicinal plants 
650 4 |a MemTTHP 
650 4 |a Micropropagation 
650 4 |a Murashige and Skoog medium 
650 4 |a ORAC 
650 4 |a PPFD 
650 4 |a Phytochemicals 
650 4 |a TE 
650 4 |a Topolins 
650 4 |a UPLC 
650 4 |a analysis of variance 
650 4 |a benzyladenine 
650 4 |a cytokinin 
650 4 |a dry weight 
650 4 |a mT 
650 4 |a mTR 
650 4 |a meta-methoxy-9-tetrahydropyran-2-yl-topolin 
650 4 |a meta-topolin 
650 4 |a meta-topolin riboside 
650 4 |a oxygen radical absorbance capacity 
650 4 |a photosynthetic photon flux density 
650 4 |a trolox equivalents 
650 4 |a ultra performance liquid chromatography 
650 7 |a Antioxidants  |2 NLM 
650 7 |a Cytokinins  |2 NLM 
650 7 |a Hydroxybenzoates  |2 NLM 
650 7 |a N(6)-(delta(2)-isopentenyl)adenine  |2 NLM 
650 7 |a 2365-40-4  |2 NLM 
650 7 |a Isopentenyladenosine  |2 NLM 
650 7 |a 7724-76-7  |2 NLM 
650 7 |a phenolic acid  |2 NLM 
650 7 |a I3P9R8317T  |2 NLM 
700 1 |a Gruz, Jiří  |e verfasserin  |4 aut 
700 1 |a Subrtová, Michaela  |e verfasserin  |4 aut 
700 1 |a Szüčová, Lucie  |e verfasserin  |4 aut 
700 1 |a Doležal, Karel  |e verfasserin  |4 aut 
700 1 |a Bairu, Michael W  |e verfasserin  |4 aut 
700 1 |a Finnie, Jeffrey F  |e verfasserin  |4 aut 
700 1 |a Van Staden, Johannes  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of plant physiology  |d 1979  |g 170(2013), 15 vom: 15. Okt., Seite 1303-8  |w (DE-627)NLM098174622  |x 1618-1328  |7 nnns 
773 1 8 |g volume:170  |g year:2013  |g number:15  |g day:15  |g month:10  |g pages:1303-8 
856 4 0 |u http://dx.doi.org/10.1016/j.jplph.2013.04.008  |3 Volltext 
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951 |a AR 
952 |d 170  |j 2013  |e 15  |b 15  |c 10  |h 1303-8