Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins

Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins

Copyright © 2013 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 170(2013), 15 vom: 15. Okt., Seite 1303-8
1. Verfasser: Aremu, Adeyemi O (VerfasserIn)
Weitere Verfasser: Gruz, Jiří, Subrtová, Michaela, Szüčová, Lucie, Doležal, Karel, Bairu, Michael W, Finnie, Jeffrey F, Van Staden, Johannes
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 2,2′-azobis(2-methylpropionamidine) dihydrochloride 2-isopentenyladenine 2iP AAPH ANOVA BA CK Conservation mehr... DW MS Medicinal plants MemTTHP Micropropagation Murashige and Skoog medium ORAC PPFD Phytochemicals TE Topolins UPLC analysis of variance benzyladenine cytokinin dry weight mT mTR meta-methoxy-9-tetrahydropyran-2-yl-topolin meta-topolin meta-topolin riboside oxygen radical absorbance capacity photosynthetic photon flux density trolox equivalents ultra performance liquid chromatography Antioxidants Cytokinins Hydroxybenzoates N(6)-(delta(2)-isopentenyl)adenine 2365-40-4 Isopentenyladenosine 7724-76-7 phenolic acid I3P9R8317T
Beschreibung
Zusammenfassung:Copyright © 2013 Elsevier GmbH. All rights reserved.
Merwilla plumbea (Lindl.) Speta is an important medicinal plant widely used in traditional medicine. We evaluated the effect of five cytokinins [benzyladenine (BA), 2-isopentenyladenine (2iP), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxy-9-tetrahydropyran-2-yl-topolin (MemTTHP)] on the level of phenolic acids and antioxidant activity of M. plumbea during the tissue culture and acclimatization stages. Two cytokinins (mT and mTR) significantly improved the antioxidant activity of tissue culture plantlets while the control plantlets were better after acclimatization. Using UPLC-MS/MS, the levels of hydroxybenzoic and hydroxycinnamic acid derivatives (phenolic acids) varied significantly during tissue culture and acclimatization, depending on the cytokinin and plant part analyzed. Vanillic acid (24.9 μg g⁻¹) detected in underground parts of tissue culture plants supplemented with BA was the most abundant phenolic acid detected. The current findings indicate that the phytochemicals together with the bioactivity during in vitro propagation of M. plumbea is influenced by the cytokinin type used and the stage of plant material collection
Beschreibung:Date Completed 13.03.2014
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1618-1328
DOI:10.1016/j.jplph.2013.04.008