Combining gene expression and genetic analyses to identify candidate genes involved in cold responses in pea

Bibliographische Detailangaben
Veröffentlicht in:	Journal of plant physiology. - 1979. - 170(2013), 13 vom: 01. Sept., Seite 1148-57
1. Verfasser:	Legrand, Sylvain (VerfasserIn)
Weitere Verfasser:	Marque, Gilles, Blassiau, Christelle, Bluteau, Aurélie, Canoy, Anne-Sophie, Fontaine, Véronique, Jaminon, Odile, Bahrman, Nasser, Mautord, Julie, Morin, Julie, Petit, Aurélie, Baranger, Alain, Rivière, Nathalie, Wilmer, Jeroen, Delbreil, Bruno, Lejeune-Hénaut, Isabelle
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Journal of plant physiology
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't C-LT C-N Champagne plants under low-temperature treatment Chilling Cold acclimation FD FT Freezing tolerance mehr... HRM LG LT N PQL Pisum sativum SSCP SSH Suppression subtractive hybridisation T-LT T-N Terese plants under low-temperature treatment WFD control/unchilled control/unchilled Champagne plants control·unchilled Terese plants freezing tolerance frost damages in controlled conditions high resolution melting curve linkage group low temperature protein quantitative locus single strand conformation polymorphism suppression subtractive hybridisation winter frost damages in the field

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier GmbH. All rights reserved. Cold stress affects plant growth and development. In order to better understand the responses to cold (chilling or freezing tolerance), we used two contrasted pea lines. Following a chilling period, the Champagne line becomes tolerant to frost whereas the Terese line remains sensitive. Four suppression subtractive hybridisation libraries were obtained using mRNAs isolated from pea genotypes Champagne and Terese. Using quantitative polymerase chain reaction (qPCR) performed on 159 genes, 43 and 54 genes were identified as differentially expressed at the initial time point and during the time course study, respectively. Molecular markers were developed from the differentially expressed genes and were genotyped on a population of 164 RILs derived from a cross between Champagne and Terese. We identified 5 candidate genes colocalizing with 3 different frost damage quantitative trait loci (QTL) intervals and a protein quantity locus (PQL) rich region previously reported. This investigation revealed the role of constitutive differences between both genotypes in the cold responses, in particular with genes related to glycine degradation pathway that could confer to Champagne a better frost tolerance. We showed that freezing tolerance involves a decrease of expression of genes related to photosynthesis and the expression of a gene involved in the production of cysteine and methionine that could act as cryoprotectant molecules. Although it remains to be confirmed, this study could also reveal the involvement of the jasmonate pathway in the cold responses, since we observed that two genes related to this pathway were mapped in a frost damage QTL interval and in a PQL rich region interval, respectively
Beschreibung:	Date Completed 03.03.2014 Date Revised 09.01.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1618-1328
DOI:	10.1016/j.jplph.2013.03.014