Soil microbial communities respond differently to three chemically defined polyphenols

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 72(2013) vom: 01. Nov., Seite 190-7
1. Verfasser:	Schmidt, Michael A (VerfasserIn)
Weitere Verfasser:	Kreinberg, Allison J, Gonzalez, Javier M, Halvorson, Jonathan J, French, Elizabeth, Bollmann, Annette, Hagerman, Ann E
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Research Support, U.S. Gov't, Non-P.H.S. AOA AOB AOX Ammonia oxidizing species CLPP Community level physiological profiling (CLPP) DGGE Denaturing gradient gel electrophoresis (DGGE) mehr... EGCg Microresp™ R(0) Soil nitrogen cycle Tannin ammonia monooxygenase gene ammonia-oxidizing archaea ammonia-oxidizing bacteria amo-A community level physiological profiling denaturing gradient gel electrophoresis epigallocatechin gallate qPCR real time quantitative polymerase chain reaction starting fluorescence Polyphenols Tannins

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier Masson SAS. All rights reserved. High molecular weight polyphenols (e.g. tannins) that enter the soil may affect microbial populations, by serving as substrates for microbial respiration or by selecting for certain microbes. In this study we examined how three phenolic compounds that represent some environmentally widespread tannins or their constituent functional groups were respired by soil microorganisms and how the compounds affected the abundance and diversity of soil bacteria and archaea, including ammonia oxidizers. An acidic, silt loam soil from a pine forest was incubated for two weeks with the monomeric phenol methyl gallate, the small polyphenol epigallocatechin gallate, or the large polyphenol oenothein B. Respiration of the polyphenols during the incubation was measured using the Microresp™ system. After incubation, metabolic diversity was determined by community level physiological profiling (CLPP), and genetic diversity was determined using denaturing gradient gel electrophoresis (DGGE) analysis on DNA extracted from the soil samples. Total microbial populations and ammonia-oxidizing populations were measured using real time quantitative polymerase chain reaction (qPCR). Methyl gallate was respired more efficiently than the higher molecular weight tannins but not as efficiently as glucose. Methyl gallate and epigallocatechin gallate selected for genetically or physiologically unique populations compared to glucose. None of the polyphenols supported microbial growth, and none of the polyphenols affected ammonia-oxidizing bacterial populations or ammonia-oxidizing archaea. Additional studies using both a wider range of polyphenols and a wider range of soils and environments are needed to elucidate the role of polyphenols in determining soil microbiological diversity
Beschreibung:	Date Completed 13.06.2014 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.03.003