Inhibition of hydroxycinnamoyl-CoA thioesterases in ginger (Zingiber officinale Rosc.) and turmeric (Curcuma longa L.) by lipase inhibitors

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 72(2013) vom: 15. Nov., Seite 46-53
1. Verfasser:	Flores-Sanchez, Isvett Josefina (VerfasserIn)
Weitere Verfasser:	Gang, David Roger
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't ACP BCoA-TE CHS CafCoA-TE CoA DTT FAS FCoA-TE mehr... Ginger HCoA-TE Hydroxycinnamoyl-CoA thioesterase NaTDC Orlistat PKS PVPP TE Turmeric Zingiberaceae acyl carrier protein benzoyl-CoA thioesterase caffeoyl-CoA thioesterase chalcone sythase coenzyme A dithiothreitol fatty acid synthase feruloyl-CoA thioesterase hydroxycinnamoyl-CoA thioesterase p-Coumaroyl-CoA thioesterase pCCoA-TE polyketide synthase polyvinylpolypyrrolidone sodium taurodeoxycholate thioesterase β-conglycinin peptides Enzyme Inhibitors Plant Proteins Esterases EC 3.1.-

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier Masson SAS. All rights reserved. Ginger (Zingiber officinale Rosc.) and turmeric (Curcuma longa L.), members of the Zingiberaceae, are widely used in traditional Asian cuisines and herbal medicine. Gingerols and diarylheptanoids, important compounds from these plants, appear to be produced by enzymes of the type III polyketide synthase class. Previous efforts to detect activity of such enzymes in tissues from these plants were only marginally successful in turmeric and completely unsuccessful in ginger because of very rapid hydrolysis of the hydroxycinnamoyl-CoA substrates (p-coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA) in these assays, presumably due to the presence of thioesterases in these tissues. In order to determine whether such thioesterase activities were specific and could be reduced so that the polyketide synthase activities could be better characterized, three inhibitors of the thioesterase domain of fatty acid synthase were tested in assays with leaf and rhizome crude protein extracts from these plants: orlistat, a reduced form of lipstatin, and peptide 1 and peptide 2 from hydrolysates of soybean β-conglycinin. Results of these analyses indicated that specific thioesterases do exist in these plants and that they could indeed be inhibited, with highest inhibition occurring with a mixture of these three compounds, leading for example to a reduction of caffeoyl-CoA hydrolysis in leaves and rhizomes of ginger by 40-fold and 27-fold, respectively
Beschreibung:	Date Completed 13.06.2014 Date Revised 09.01.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.02.012