Effects of urea on selectivity and protein-ligand interactions in multimodal cation exchange chromatography

Nuclear magnetic resonance (NMR) and molecular dynamics (MD) simulations were employed in concert with chromatography to provide insight into the effect of urea on protein-ligand interactions in multimodal (MM) chromatography. Chromatographic experiments with a protein library in ion exchange (IEX)...

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Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 29(2013), 1 vom: 08. Jan., Seite 158-67
Auteur principal: Holstein, Melissa A (Auteur)
Autres auteurs: Parimal, Siddharth, McCallum, Scott A, Cramer, Steven M
Format: Article en ligne
Langue:English
Publié: 2013
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Ligands Proteins Ubiquitin Urea 8W8T17847W