Proteolytic Activities in Alfalfa (Medicago sativa L.) Leaves
Copyright © 1984 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved.
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 116(1984), 2 vom: 15. Sept., Seite 133-45 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1984
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article |
| Zusammenfassung: | Copyright © 1984 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved. Autodigestion in alfalfa (Medicago sativa L. cv Messe) leaves takes place at a higher rate in the acidic range of pH suggesting that acid proteases are probably involved in protein breakdown. Several lines of evidence show that alfalfa leaves contain various proteolytic activities. A carboxypeptidase active on CBZ-Phe-Met and on other CBZ-dipeptides with a pH optimum of 5.5 has been partially purified by CM-cellulose chromatography. Another acid protease, probably endopeptidase, is active on hemoglobin with an optimum at pH 4.5; perhaps it is not a single protease since it shows another peak of activity at pH 7-7.5. In the neutral-alkaline range of pH there are at least three peptidases active on naphthylamides. One of these, active on Lys-NA, is most active in very young leaves and probably is aSH dependent protease |
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| Beschreibung: | Date Completed 03.12.2012 Date Revised 30.09.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/S0176-1617(84)80070-X |