Proteolytic Activities in Alfalfa (Medicago sativa L.) Leaves

Copyright © 1984 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 116(1984), 2 vom: 15. Sept., Seite 133-45
1. Verfasser: Scalet, M (VerfasserIn)
Weitere Verfasser: Alpi, A, Picciarelli, P
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 1984
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article
LEADER 01000naa a22002652 4500
001 NLM223016640
003 DE-627
005 20231224055715.0
007 cr uuu---uuuuu
008 231224s1984 xx |||||o 00| ||eng c
024 7 |a 10.1016/S0176-1617(84)80070-X  |2 doi 
028 5 2 |a pubmed24n0743.xml 
035 |a (DE-627)NLM223016640 
035 |a (NLM)23195048 
035 |a (PII)S0176-1617(84)80070-X 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Scalet, M  |e verfasserin  |4 aut 
245 1 0 |a Proteolytic Activities in Alfalfa (Medicago sativa L.) Leaves 
264 1 |c 1984 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 03.12.2012 
500 |a Date Revised 30.09.2020 
500 |a published: Print-Electronic 
500 |a Citation Status PubMed-not-MEDLINE 
520 |a Copyright © 1984 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved. 
520 |a Autodigestion in alfalfa (Medicago sativa L. cv Messe) leaves takes place at a higher rate in the acidic range of pH suggesting that acid proteases are probably involved in protein breakdown. Several lines of evidence show that alfalfa leaves contain various proteolytic activities. A carboxypeptidase active on CBZ-Phe-Met and on other CBZ-dipeptides with a pH optimum of 5.5 has been partially purified by CM-cellulose chromatography. Another acid protease, probably endopeptidase, is active on hemoglobin with an optimum at pH 4.5; perhaps it is not a single protease since it shows another peak of activity at pH 7-7.5. In the neutral-alkaline range of pH there are at least three peptidases active on naphthylamides. One of these, active on Lys-NA, is most active in very young leaves and probably is aSH dependent protease 
650 4 |a Journal Article 
700 1 |a Alpi, A  |e verfasserin  |4 aut 
700 1 |a Picciarelli, P  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of plant physiology  |d 1979  |g 116(1984), 2 vom: 15. Sept., Seite 133-45  |w (DE-627)NLM098174622  |x 1618-1328  |7 nnns 
773 1 8 |g volume:116  |g year:1984  |g number:2  |g day:15  |g month:09  |g pages:133-45 
856 4 0 |u http://dx.doi.org/10.1016/S0176-1617(84)80070-X  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 116  |j 1984  |e 2  |b 15  |c 09  |h 133-45