Continuous enzyme-coupled assay of phosphate- or pyrophosphate-releasing enzymes
A coupled enzyme assay able to monitor the kinetics of reactions catalyzed by phosphate- or pyrophosphate-releasing enzymes is presented here. The assay is based on the concerted action of inorganic pyrophosphatase (PPase), purine nucleoside phosphorylase (PNPase), and xanthine oxidase (XOD). In the...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 53(2012), 2 vom: 23. Aug., Seite 99-103
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| 1. Verfasser: |
Suárez, Antonio S Guillén
(VerfasserIn) |
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| Weitere Verfasser: |
Stefan, Alessandra,
Lemma, Silvia,
Conte, Emanuele,
Hochkoeppler, Alejandro |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Phosphates
Hypoxanthine
2TN51YD919
Inosine
5A614L51CT
DNA
9007-49-2
calf thymus DNA
91080-16-9
mehr...
Xanthine Oxidase
EC 1.17.3.2
Purine-Nucleoside Phosphorylase
EC 2.4.2.1
DNA Polymerase I
EC 2.7.7.7
DNA-Directed DNA Polymerase
Inorganic Pyrophosphatase
EC 3.6.1.1 |
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