Effective flow cytometric phenotyping of cells using minimal amounts of antibody

Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproduc...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:	BioTechniques. - 1993. - 53(2012), 1 vom: 28. Juli, Seite 57-60
1. Verfasser:	Sharma, Deepak (VerfasserIn)
Weitere Verfasser:	Eichelberg, Mark R, Haag, Jill D, Meilahn, Amanda L, Muelbl, Matthew J, Schell, Kathy, Smits, Bart M G, Gould, Michael N
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2012
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, N.I.H., Extramural Antigens, CD

Beschreibung
Zusammenfassung:	Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens
Beschreibung:	Date Completed 05.11.2012 Date Revised 21.10.2021 published: Print Citation Status MEDLINE
ISSN:	1940-9818
DOI:	10.2144/0000113854