Effective flow cytometric phenotyping of cells using minimal amounts of antibody
Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproduc...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 53(2012), 1 vom: 28. Juli, Seite 57-60
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| 1. Verfasser: |
Sharma, Deepak
(VerfasserIn) |
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| Weitere Verfasser: |
Eichelberg, Mark R,
Haag, Jill D,
Meilahn, Amanda L,
Muelbl, Matthew J,
Schell, Kathy,
Smits, Bart M G,
Gould, Michael N |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Antigens, CD |
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