Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels
An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-le...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 0(2012), 0 vom: 06. Mai, Seite 1-6
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| 1. Verfasser: |
Domanski, Michal
(VerfasserIn) |
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| Weitere Verfasser: |
Molloy, Kelly,
Jiang, Hua,
Chait, Brian T,
Rout, Michael P,
Jensen, Torben Heick,
LaCava, John |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article |
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