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231224s2012 xx |||||o 00| ||eng c |
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7 |
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|a 10.2144/000113864
|2 doi
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| 041 |
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|a eng
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| 100 |
1 |
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|a Domanski, Michal
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels
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| 264 |
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1 |
|c 2012
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Revised 21.10.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented
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| 650 |
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|a Journal Article
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1 |
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|a Molloy, Kelly
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chait, Brian T
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rout, Michael P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jensen, Torben Heick
|e verfasserin
|4 aut
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| 700 |
1 |
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|a LaCava, John
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 0(2012), 0 vom: 06. Mai, Seite 1-6
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:0
|g year:2012
|g number:0
|g day:06
|g month:05
|g pages:1-6
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| 856 |
4 |
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