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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2012.04.004
|2 doi
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|a pubmed25n0726.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Kim, Min-Jung
|e verfasserin
|4 aut
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|a Identification of nuclear localization signal in ASYMMETRIC LEAVES2-LIKE18/LATERAL ORGAN BOUNDARIES DOMAIN16 (ASL18/LBD16) from Arabidopsis
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 07.12.2012
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|a Date Revised 10.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2012 Elsevier GmbH. All rights reserved.
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|a The ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) gene family encodes proteins harboring a conserved plant-specific LOB domain. The LOB domain contains a four-Cys motif, a Gly-Ala-Ser (GAS) block, and a Leu-zipper-like coiled-coil motif. The ASL/LBD proteins are a unique class of transcription factors that play roles in lateral organ development of plants. Although the ASL/LBD proteins are localized in the nucleus, no consensus sequence for the nuclear localization of the ASL/LBD proteins could be found. In the present study, we determined the motifs responsible for the nuclear localization of ASL18/LBD16 by using protoplast transfection assays with a variety N- or C-terminal deletion polypeptide fragments and the polypeptides harboring changes in basic amino acids that are fused to enhanced green fluorescent protein. The results demonstrated that ASL18/LBD16 harbors two distinct domains comprising an atypical nuclear localization signal (NLS) with basic amino acid residues in the coiled-coil motif and a monopartite-like NLS in the C-terminal region for nuclear targeting
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis Proteins
|2 NLM
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|a LBD18 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Nuclear Localization Signals
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 700 |
1 |
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|a Kim, Jungmook
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 169(2012), 12 vom: 15. Aug., Seite 1221-6
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnas
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| 773 |
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|g volume:169
|g year:2012
|g number:12
|g day:15
|g month:08
|g pages:1221-6
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|u http://dx.doi.org/10.1016/j.jplph.2012.04.004
|3 Volltext
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