Identification of nuclear localization signal in ASYMMETRIC LEAVES2-LIKE18/LATERAL ORGAN BOUNDARIES DOMAIN16 (ASL18/LBD16) from Arabidopsis

Bibliographische Detailangaben
Veröffentlicht in:	Journal of plant physiology. - 1979. - 169(2012), 12 vom: 15. Aug., Seite 1221-6
1. Verfasser:	Kim, Min-Jung (VerfasserIn)
Weitere Verfasser:	Kim, Jungmook
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2012
Zugriff auf das übergeordnete Werk:	Journal of plant physiology
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Arabidopsis Proteins LBD18 protein, Arabidopsis Nuclear Localization Signals Transcription Factors

Beschreibung
Zusammenfassung:	Copyright © 2012 Elsevier GmbH. All rights reserved. The ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) gene family encodes proteins harboring a conserved plant-specific LOB domain. The LOB domain contains a four-Cys motif, a Gly-Ala-Ser (GAS) block, and a Leu-zipper-like coiled-coil motif. The ASL/LBD proteins are a unique class of transcription factors that play roles in lateral organ development of plants. Although the ASL/LBD proteins are localized in the nucleus, no consensus sequence for the nuclear localization of the ASL/LBD proteins could be found. In the present study, we determined the motifs responsible for the nuclear localization of ASL18/LBD16 by using protoplast transfection assays with a variety N- or C-terminal deletion polypeptide fragments and the polypeptides harboring changes in basic amino acids that are fused to enhanced green fluorescent protein. The results demonstrated that ASL18/LBD16 harbors two distinct domains comprising an atypical nuclear localization signal (NLS) with basic amino acid residues in the coiled-coil motif and a monopartite-like NLS in the C-terminal region for nuclear targeting
Beschreibung:	Date Completed 07.12.2012 Date Revised 10.03.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1618-1328
DOI:	10.1016/j.jplph.2012.04.004