Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells
Copyright © 2012 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 55(2012) vom: 03. Juni, Seite 77-84 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2012
|
| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Anthocyanins Culture Media Phosphates Plant Proteins Phenylalanine 47E5O17Y3R Glucosyltransferases EC 2.4.1.- flavonol 3-O-glucosyltransferase |
| Zusammenfassung: | Copyright © 2012 Elsevier Masson SAS. All rights reserved. The effect of phosphate (Pi) starvation on anthocyanin biosynthesis was examined in suspension-cultured grape cells derived from Bailey Alicante A grapes. Cyanindin-3-O-glucoside, peonidin-3-O-glucoside, cyanidin-3-O-(6-O״-p-coumaroyl)-glucoside and peonidin-3-O-(6-O״-p-coumaroyl)-glucoside were detected in cells in both the complete (+Pi)- and Pi-deficient Murashige-Skoog (-Pi) medium but accumulation was much more substantial cells in -Pi cultures. Highest incorporation of [ring-(14)C]phenylalanine into anthocyanins was found at days 3-14 in the cells in -Pi medium. Time-course expression profiles of anthocyanin biosynthetic enzyme genes and a regulatory gene were examined after cells were transferred to the +Pi and -Pi medium. The transcript levels of UFGT and VvmybA1 in -Pi cells were higher than in +Pi cells 24 h after transfer coinciding with the start of anthocyanin accumulation in -Pi cells |
|---|---|
| Beschreibung: | Date Completed 21.09.2012 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2012.03.009 |