Label-free ultrasensitive detection of human telomerase activity using porphyrin-functionalized graphene and electrochemiluminescence technique
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
| Veröffentlicht in: | Advanced materials (Deerfield Beach, Fla.). - 1998. - 24(2012), 18 vom: 08. Mai, Seite 2447-52 |
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| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | Advanced materials (Deerfield Beach, Fla.) |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Oxides Porphyrins Graphite 7782-42-5 Telomerase EC 2.7.7.49 |
| Zusammenfassung: | Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Using porphyrin-functionalized graphene, we construct a PCR-free, low-cost, rapid, and electrochemiluminenscence (ECL) assay for detection of telomerase activity that has been demonstrated in six different cell lines and can be used as initial screening of G-quadruplex DNA binding agents and telomerase inhibitors. This ECL sensor shows highly sensitive for detection of telomerase with the detection limit as low as 10 HeLa cells mL(-1) |
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| Beschreibung: | Date Completed 04.09.2012 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1521-4095 |
| DOI: | 10.1002/adma.201200412 |