Isolation and characterization of terpene synthases potentially involved in flavor development of ripening olive (Olea europaea) fruits
Copyright © 2012 Elsevier GmbH. All rights reserved.
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 169(2012), 9 vom: 15. Juni, Seite 908-14 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Comparative Study Journal Article Research Support, Non-U.S. Gov't Ethylenes Monoterpenes Plant Growth Regulators ethylene 91GW059KN7 Alkyl and Aryl Transferases EC 2.5.- mehr... |
| Zusammenfassung: | Copyright © 2012 Elsevier GmbH. All rights reserved. The flavor and taste of fruits are often determined by terpenes. We identified three cDNAs encoding putative terpene synthases from olive fruits of cv. Frantoio and Grignano. Heterologous expression in a bacterial system demonstrated that one of the terpene synthases, OeGES1, was an active monoterpene synthase that converted geranyl diphosphate to the monoterpene alcohol geraniol. The transcript accumulation pattern of this gene showed a peak during fruit ripening in both genotypes, indicating that the enzyme may be involved in the production of monoterpene flavor compounds in olive fruit. Although the putative terpene synthases OeTPS2 and OeTPS3 clustered with α-farnesene synthases and angiosperm monoterpene synthases, no detectable in vitro activity was found after expression in a bacterial system. Nevertheless, their transcripts sharply accumulated during fruit ripening starting from véraison |
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| Beschreibung: | Date Completed 27.08.2012 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/j.jplph.2012.01.021 |