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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ers021
|2 doi
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|a pubmed24n0718.xml
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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1 |
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|a Lukasik-Shreepaathy, Ewa
|e verfasserin
|4 aut
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|a Protein-protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 07.09.2012
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Plant resistance proteins (R) are involved in pathogen recognition and subsequent initiation of defence responses. Their activity is regulated by inter- and intramolecular interactions. In a yeast two-hybrid screen two clones (I2I-1 and I2I-2) specifically interacting with I-2, a Fusarium oxysporum f. sp. lycopersici resistance protein of the CC-NB-LRR family, were identified. Sequence analysis revealed that I2I-1 belongs to the Formin gene family (SlFormin) whereas I2I-2 has homology to translin-associated protein X (SlTrax). SlFormin required only the N-terminal CC I-2 domain for binding, whereas SlTrax required both I-2 CC and part of the NB-ARC domain. Tomato plants stably silenced for these interactors were not compromised in I-2-mediated disease resistance. When extended or mutated forms of I-2 were used as baits, distinct and often opposite, interaction patterns with the two interactors were observed. These interaction patterns correlated with the proposed activation state of I-2 implying that active and inactive R proteins adopt distinct conformations. It is concluded that the yeast two hybrid system can be used as a proxy to monitor these different conformational states
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|a Journal Article
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|a Mutant Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Vossen, Jack H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tameling, Wladimir I L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a de Vroomen, Marianne J
|e verfasserin
|4 aut
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| 700 |
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|a Cornelissen, Ben J C
|e verfasserin
|4 aut
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| 700 |
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|a Takken, Frank L W
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 63(2012), 8 vom: 15. Mai, Seite 3047-60
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:63
|g year:2012
|g number:8
|g day:15
|g month:05
|g pages:3047-60
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|u http://dx.doi.org/10.1093/jxb/ers021
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