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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1111/j.1939-165X.2011.00384.x
|2 doi
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|a eng
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| 100 |
1 |
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|a Mudge, Margaret C
|e verfasserin
|4 aut
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|a Post-transfusion survival of biotin-labeled allogeneic RBCs in adult horses
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 02.08.2012
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|a Date Revised 20.10.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2012 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Post-transfusion survival of allogeneic RBCs has been reported to be much shorter in horses than in other species. We hypothesized that post-transfusion survival of biotinylated allogeneic equine RBCs would be greater than the survival previously reported from studies using radioactive RBC-labeling techniques
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|a OBJECTIVE: The study objective was to determine post-transfusion survival of N-hydroxysuccinimide (NHS)-biotin-labeled allogeneic equine RBCs transfused into adult horses
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|a METHODS: Horses were adults and included 5 donors and 5 recipients. All horses were blood-typed, and donors were paired with recipients based upon blood type and crossmatch results. Donor blood was collected in a volume of 4 L into citrate phosphate dextrose adenine-1 and stored for 24 hours, labeled with NHS-biotin, and re-infused into recipients. Post-transfusion blood samples were collected at 15 minutes and at 1, 2, 3, 5, 7, 14, 21, 28, and 35 days. Biotin-labeled RBCs were detected by flow cytometry using streptavidin-phycoerythrin. Post-transfusion survival at 24 hours, lifespan, and half-life of biotinylated RBCs were determined
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| 520 |
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|a RESULTS: Mean ± SD survival of biotinylated RBCs at 24 hours post-transfusion was 95 ± 24%; the mean lifespan of transfused allogeneic RBCs was 39 days based on calculation of a linear regression survival curve, and mean post-transfusion RBC half-life was 20 days
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|a CONCLUSIONS: Post-transfusion survival of 24-hour stored equine allogeneic RBCs was greater than previously reported but less than that observed for other companion animal species. Mechanisms for the relatively short post-transfusion lifespan of allogeneic equine RBCs remain unknown and warrant further study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Walker, Naomi J
|e verfasserin
|4 aut
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|a Borjesson, Dori L
|e verfasserin
|4 aut
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| 700 |
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|a Librach, Fred
|e verfasserin
|4 aut
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| 700 |
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|a Johns, Jennifer L
|e verfasserin
|4 aut
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| 700 |
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|a Owens, Sean D
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 41(2012), 1 vom: 18. März, Seite 56-62
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|x 1939-165X
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|g year:2012
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|g month:03
|g pages:56-62
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