Post-transfusion survival of biotin-labeled allogeneic RBCs in adult horses
© 2012 American Society for Veterinary Clinical Pathology.
Veröffentlicht in: | Veterinary clinical pathology. - 1975. - 41(2012), 1 vom: 18. März, Seite 56-62 |
---|---|
1. Verfasser: | |
Weitere Verfasser: | , , , , |
Format: | Online-Aufsatz |
Sprache: | English |
Veröffentlicht: |
2012
|
Zugriff auf das übergeordnete Werk: | Veterinary clinical pathology |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't |
Zusammenfassung: | © 2012 American Society for Veterinary Clinical Pathology. BACKGROUND: Post-transfusion survival of allogeneic RBCs has been reported to be much shorter in horses than in other species. We hypothesized that post-transfusion survival of biotinylated allogeneic equine RBCs would be greater than the survival previously reported from studies using radioactive RBC-labeling techniques OBJECTIVE: The study objective was to determine post-transfusion survival of N-hydroxysuccinimide (NHS)-biotin-labeled allogeneic equine RBCs transfused into adult horses METHODS: Horses were adults and included 5 donors and 5 recipients. All horses were blood-typed, and donors were paired with recipients based upon blood type and crossmatch results. Donor blood was collected in a volume of 4 L into citrate phosphate dextrose adenine-1 and stored for 24 hours, labeled with NHS-biotin, and re-infused into recipients. Post-transfusion blood samples were collected at 15 minutes and at 1, 2, 3, 5, 7, 14, 21, 28, and 35 days. Biotin-labeled RBCs were detected by flow cytometry using streptavidin-phycoerythrin. Post-transfusion survival at 24 hours, lifespan, and half-life of biotinylated RBCs were determined RESULTS: Mean ± SD survival of biotinylated RBCs at 24 hours post-transfusion was 95 ± 24%; the mean lifespan of transfused allogeneic RBCs was 39 days based on calculation of a linear regression survival curve, and mean post-transfusion RBC half-life was 20 days CONCLUSIONS: Post-transfusion survival of 24-hour stored equine allogeneic RBCs was greater than previously reported but less than that observed for other companion animal species. Mechanisms for the relatively short post-transfusion lifespan of allogeneic equine RBCs remain unknown and warrant further study |
---|---|
Beschreibung: | Date Completed 02.08.2012 Date Revised 20.10.2016 published: Print-Electronic Citation Status MEDLINE |
ISSN: | 1939-165X |
DOI: | 10.1111/j.1939-165X.2011.00384.x |